Limiting immunopathology: Interaction between carotenoids and enzymatic antioxidant defences

• Published in: Developmental and comparative immunology 2015-04, Vol.49 (2), p.278-281
• Main Authors: Babin, A., Saciat, C., Teixeira, M., Troussard, J.-P., Motreuil, S., Moreau, J., Moret, Y.
• Format: Article
• Language: English
• Subjects: Amphipoda - immunology; Animals; Antioxidant; Antioxidants - metabolism; Biodiversity and Ecology; Carotenoids; Carotenoids - metabolism; Catalase - metabolism; Crustacean; Dietary Supplements; Ecological immunology; Environmental Sciences; Gammarus pulex; Hemolymph - chemistry; Hemolymph - cytology; Hemolymph - immunology; Immuno-stimulants; Immunology; Immunopathology; Inflammation - immunology; Life Sciences; Male; Oxidative Stress; Reactive Nitrogen Species - immunology; Reactive Nitrogen Species - metabolism; Reactive Oxygen Species - immunology; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2014.12.007

•The interaction between carotenoids and antioxidant enzymes is tested in a crustacean.•Increased carotenoid concentrations in the haemolymph stimulated immune defences.•Upon immune activation, concentrations of carotenoids and haemocytes decreased.•Stored carotenoids down-regulated SOD activity but promoted CAT activity.•Dietary carotenoids interact in a specific way with endogenous antioxidant enzymes. The release of reactive oxygen and nitrogen species (ROS and RNS) during the inflammatory response generates damages to host tissues, referred to as immunopathology, and is an important factor in ecological immunology. The integrated antioxidant system, comprising endogenous antioxidant enzymes (e.g. superoxide dismutase SOD, and catalase CAT) and dietary antioxidants (e.g. carotenoids), helps to cope with immune-mediated oxidative stress. Crustaceans store large amounts of dietary carotenoids for yet unclear reasons. While being immunostimulants and antioxidants, the interaction of these pigments with antioxidant enzymes remains unclear. Here, we tested the interaction between dietary supplementation with carotenoids and immune challenge on immune defences and the activity of the antioxidant enzymes SOD and CAT, in the amphipod crustacean Gammarus pulex. Dietary supplementation increased the concentrations of circulating carotenoids and haemocytes in the haemolymph, while the immune response induced the consumption of circulating carotenoids and a drop of haemocyte density. Interestingly, supplemented gammarids exhibited down-regulated SOD activity but high CAT activity compared to control ones. Our study reveals specific interactions of dietary carotenoids with endogenous antioxidant enzymes, and further underlines the potential importance of carotenoids in the evolution of immunity and/or of antioxidant mechanisms in crustaceans.