Linkage and physical mapping of rat microsatellites derived from minisatellite loci

Microsatellite and minisatellite markers are the major components of genetic linkage maps. Microsatellite markers are spread along the genome, whereas minisatellites show a non-random chromosomal distribution, different from species to species. In human, they are predominantly clustered in the subte...

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Bibliographic Details
Published in:Mammalian genome 1999-04, Vol.10 (4), p.405-409
Main Authors: Giraudeau, F, Apiou, F, Amarger, V, Kaisaki, P J, Bihoreau, M T, Lathrop, M, Vergnaud, G, Gauguier, D
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
DNA, Satellite - genetics
Genetic Linkage
Genetics
Life Sciences
Microsatellite Repeats - genetics
Rats
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Summary:Microsatellite and minisatellite markers are the major components of genetic linkage maps. Microsatellite markers are spread along the genome, whereas minisatellites show a non-random chromosomal distribution, different from species to species. In human, they are predominantly clustered in the subtelomeric regions, defined as the terminal cytogenetic band (Royle et al. 1988; Wells et al. 1989; Vergnaud et al. 1991; Amarger et al. 1998), whereas in other species this bias towards chromosome ends does not seem to occur (Amarger et al. 1998). The differential chromosomal mapping of minisatellites among species supports the hypothesis that minisatellite loci indicate internalization events of telomeric domains (as the result, for instance, of chromosome ends fusions). The integration of minisatellite-associated loci in genetic maps at a high resolution is necessary to efficiently test this hypothesis. Minisatellite location could then indeed provide important information on the definition of boundaries between conserved synteny groups and on the precise localization of chromosomal rearrangements involving chromosome ends that arose in mammalian genomes. Minisatellite mapping requires Southern blotting, which is not used in large-scale genetic mapping projects. To circumvent this difficulty, we have characterized 51 microsatellites from our initial minisatellite-containing cosmids. We report here the integration of 50 microsatellites identified in either a dense linkage map of the rat (Bihoreau et al. 1997) or a radiation hybrid (RH) map together with their chromosomal assignment as deduced by fluorescent in situ hybridization (FISH) analysis.
ISSN:0938-8990
1432-1777
DOI:10.1007/s003359901012