Nitric oxide participates at the first steps of Apis mellifera cellular immune activation in response to non-self recognition

The honey bee Apis mellifera (Hymenoptera) is being affected by many diseases. The elimination of organisms in the insect hemocoel requires hemocytes recognition and response to the invader. After recognizing a surface as “foreign,” hemocytes “spread.” Spreading on glass surfaces by insect hemocytes...

Full description

Saved in:
Bibliographic Details
Published in:Apidologie 2013-09, Vol.44 (5), p.575-585
Main Authors: Negri, Pedro, Maggi, Matías, Correa-Aragunde, Natalia, Brasesco, Constanza, Eguaras, Martín, Lamattina, Lorenzo
Format: Article
Language:English
Subjects:
Agricultural sciences
Animal biology
Animal production studies
Animal productions
Apiculture
Apis mellifera
Biochemistry. Physiology. Immunology
Biodiversity
Biological and medical sciences
Biomedical and Life Sciences
Ecology, environment
Entomology
Fundamental and applied biological sciences. Psychology
glass
hemocoel
hemocytes
honey bees
immune response
Insecta
Invertebrate Zoology
Invertebrates
Life Sciences
nitric oxide
nitroprusside
Original Article
Physiology. Development
Terrestrial animal productions
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The honey bee Apis mellifera (Hymenoptera) is being affected by many diseases. The elimination of organisms in the insect hemocoel requires hemocytes recognition and response to the invader. After recognizing a surface as “foreign,” hemocytes “spread.” Spreading on glass surfaces by insect hemocytes is used as a measure of immune activation. Nitric oxide (NO) is a signaling and immune effector molecule in response to microbial infection that has been proposed as a key molecule in invertebrate immunity. The participation of NO in the hemocytic response of A. mellifera upon recognition of non-self is herein analyzed. Glass-adherent hemocytes produce large amounts of NO. Treatment with NO donor sodium nitroprusside enhanced hemocyte spreading, while NO scavenger carboxyPTIO reduced hemocyte immune activation. These results are indicative of NO participation at the beginning of A. mellifera immune response to non-self.
ISSN:0044-8435
1297-9678
DOI:10.1007/s13592-013-0207-8