Glycosylation-related genes are variably expressed depending on the differentiation state of a bioaminergic neuronal cell line: implication for the cellular prion protein

A striking feature of the cellular prion protein (PrPC) is the heterogeneity of its glycoforms, whose contribution to PrPC function has yet to be defined. Using the 1C11 neuronal bioaminergic differentiation model and a glycomics approach, we show here a correlation between differential PrPC N-glyco...

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Bibliographic Details
Published in:Glycoconjugate journal 2009-05, Vol.26 (4), p.477-493
Main Authors: Ermonval, Myriam, Petit, Daniel, Le Duc, Aurélien, Kellermann, Odile, Gallet, Paul-François
Format: Article
Language:English
Subjects:
Biochemistry
Biogenic Amines - metabolism
Biomedical and Life Sciences
Cell Differentiation - genetics
Cell Line
Cellular biology
Electrophoresis
Enzyme-Linked Immunosorbent Assay
Gene expression
Gene Expression Profiling
Gene Expression Regulation
glycoconjugates
Glycomics
Glycosaminoglycans - biosynthesis
Glycosylation
Glycotranscriptome
Life Sciences
N-glycosylation
Neuronal differentiation
Neurons
Neurons - cytology
Neurons - metabolism
Pathology
Phylogeny
Prions
Prions - metabolism
Proteins
PrPC
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - metabolism
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Summary:A striking feature of the cellular prion protein (PrPC) is the heterogeneity of its glycoforms, whose contribution to PrPC function has yet to be defined. Using the 1C11 neuronal bioaminergic differentiation model and a glycomics approach, we show here a correlation between differential PrPC N-glycosylations in 1C11⁵⁻HT serotonergic and 1C11NE noradrenergic cells compared to their 1C11 precursor cells and a variation of the glycogenome expression status in these cells. In particular, expression of genes involved in N-glycan synthesis or in the modeling of chondroitin and heparan sulfate proteoglycans appeared to be modulated. Our results highlight that, the expression of glycosylation-related genes is regulated during bioaminergic neuronal differentiation, consistent with a participation of glycoconjugates in neuronal development and plasticity. A neuronal regulation of glycosylation processes may have direct implications on some neurospecific functions of PrPC and may participate in specific brain targeting of prion strains.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-008-9198-5