Transcriptomic Response of Lactococcus lactis in Mixed Culture with Staphylococcus aureus

• Published in: Applied and Environmental Microbiology 2009-07, Vol.75 (13), p.4473-4482
• Main Authors: Nouaille, Sébastien, Even, Sergine, Charlier, Cathy, Le Loir, Yves, Cocaign-Bousquet, Muriel, Loubière, Pascal
• Format: Article
• Language: English
• Subjects: Amino acids; Antibiosis; Bacterial Proteins - biosynthesis; Biological and medical sciences; Coculture Techniques; Ecology, environment; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genetics and Molecular Biology; Glucose; Lactococcus lactis; Lactococcus lactis - physiology; Life Sciences; Metabolic Networks and Pathways - genetics; Microbiology; Staphylococcus aureus - physiology; Staphylococcus infections; Studies
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.02653-08

The mechanisms of interaction between Lactococcus lactis and the food pathogen Staphylococcus aureus are of crucial importance, as one major role of lactic acid bacteria (LAB) in fermented foods is to inhibit undesirable and pathogenic flora. It was never questioned if the presence of a pathogen can actively modify the gene expression patterns of LAB in a shared environment. In this study, transcriptome and biochemical analyses were combined to assess the dynamic response of L. lactis in a mixed culture with S. aureus. The presence of S. aureus hardly affected the growth of L. lactis but dramatically modified its gene expression profile. The main effect was related to earlier carbon limitation and a concomitantly lower growth rate in the mixed culture due to the consumption of glucose by both species. More specific responses involved diverse cellular functions. Genes associated with amino acid metabolism, ion transport, oxygen response, menaquinone metabolism, and cell surface and phage expression were differentially expressed in the mixed culture. This study led to new insights into possible mechanisms of interaction between L. lactis and S. aureus. Moreover, new and unexpected effects of L. lactis on the virulence of S. aureus were discovered, as described elsewhere (S. Even, C. Charlier, S. Nouaille, N. L. Ben Zakour, M. Cretenet, F. J. Cousin, M. Gautier, M. Cocaign-Bousquet, P. Loubière, and Y. Le Loir, Appl. Environ. Microbiol. 75:4459-4472, 2009).