Biochemical Investigation of the Formation of Three-Dimensional Networks from DNA-Grafted Large Silica Particles

DNA is used to rationally build up networks of silica nanoparticles (SiNPs) based on the molecular recognition properties of complementary sequences. Network self-assembly is controlled from DNA covalently grafted at the surface of chemically modified SiNPs. Two strategies are compared, where grafte...

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Bibliographic Details
Published in:Langmuir 2012-01, Vol.28 (4), p.2156-2165
Main Authors: Wu, Jiangyu, Silvent, Jérémie, Coradin, Thibaud, Aimé, Carole
Format: Article
Language:English
Subjects:
Base Sequence
Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials
Chemical Sciences
DNA - chemistry
DNA - genetics
Electrophoresis
Material chemistry
Microscopy, Electron
Models, Molecular
Nanoparticles - chemistry
Nucleic Acid Hybridization
Particle Size
Silicon Dioxide - chemistry
Spectrum Analysis
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Summary:DNA is used to rationally build up networks of silica nanoparticles (SiNPs) based on the molecular recognition properties of complementary sequences. Network self-assembly is controlled from DNA covalently grafted at the surface of chemically modified SiNPs. Two strategies are compared, where grafted DNA sequences are designed in a three-strand system using noncomplementary sequences and an extra DNA linker, or in a two-strand approach for direct hybridization. In this paper, both systems are compared in terms of DNA hybridization stability, network size, and three-dimensional organization using a combination of dynamic light scattering and electron microscopy. The observed differences are discussed in terms of hybridization interactions between DNA sequences in particle-free systems through fluorescence, circular dichroism, and UV spectroscopy techniques.
ISSN:0743-7463
1520-5827
DOI:10.1021/la2037372