Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

Advanced tools for cell imaging are of great interest for the detection, localization, and quantification of molecular biomarkers of cancer or infection. We describe a novel photopolymerization method to coat quantum dots (QDs) with polymer shells, in particular, molecularly imprinted polymers (MIPs...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2016-07, Vol.55 (29), p.8244-8248
Main Authors: Panagiotopoulou, Maria, Salinas, Yolanda, Beyazit, Selim, Kunath, Stephanie, Duma, Luminita, Prost, Elise, Mayes, Andrew G., Resmini, Marina, Tse Sum Bui, Bernadette, Haupt, Karsten
Format: Article
Language:English
Subjects:
Binding
Biocompatibility
bioimaging
Biomarkers
Cancer
Cleavage
Coating
Core-shell particles
Fluorescence
Imaging
Imprinted polymers
Infections
Keratinocytes
Labels
Life Sciences
Localization
Marking
molecularly imprinted polymers
Multiplexing
N-Acetylneuraminic acid
nanocomposites
Photopolymerization
Polymer coatings
Polymers
Quantum dots
Shells
synthetic receptors
Ultraviolet radiation
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Summary:Advanced tools for cell imaging are of great interest for the detection, localization, and quantification of molecular biomarkers of cancer or infection. We describe a novel photopolymerization method to coat quantum dots (QDs) with polymer shells, in particular, molecularly imprinted polymers (MIPs), by using the visible light emitted from QDs excited by UV light. Fluorescent core–shell particles specifically recognizing glucuronic acid (GlcA) or N‐acetylneuraminic acid (NANA) were prepared. Simultaneous multiplexed labeling of human keratinocytes with green QDs conjugated with MIP‐GlcA and red QDs conjugated with MIP‐NANA was demonstrated by fluorescence imaging. The specificity of binding was verified with a non‐imprinted control polymer and by enzymatic cleavage of the terminal GlcA and NANA moieties. The coating strategy is potentially a generic method for the functionalization of QDs to address a much wider range of biocompatibility and biorecognition issues. Labels to tell them apart: The visible light emitted from quantum dots excited by UV light was used to photopolymerize a molecularly imprinted polymer (MIP) shell around the QDs. The use of different quantum dots with MIP shells that recognize glucuronic acid (green) or N‐acetylneuraminic acid (red) enabled the multiplexed labeling and imaging of keratinocytes. The labels could be differentiated and quantified on and in the cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201601122