Simultaneous vitality and DNA‐fragmentation measurement in spermatozoa of smokers and non‐smokers

Background Because cigarette smoke is a powerful ROS producer, we hypothesized that the spermatozoa of smokers would be more at risk of having increased DNA fragmentation than spermatozoa of non‐smoking men. Methods A cross‐sectional study was performed on consenting smokers and non‐smokers, consult...

Full description

Saved in:
Bibliographic Details
Published in:Cytometry. Part B, Clinical cytometry Clinical cytometry, 2015-03, Vol.88 (2), p.120-124
Main Authors: De Bantel, A., Fleury‐Feith, J., Poirot, C., Berthaut, I., Garcin, C., Landais, P., Ravel, C.
Format: Article
Language:English
Subjects:
Adolescent
Adult
cell vitality
Cross-Sectional Studies
DNA Fragmentation
Flow Cytometry - methods
Human health and pathology
Humans
Life Sciences
Male
Middle Aged
Semen Analysis - methods
Smoking - adverse effects
Smoking - pathology
spermatozoa
Spermatozoa - pathology
Spermatozoa - physiology
tobacco
Young Adult
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Because cigarette smoke is a powerful ROS producer, we hypothesized that the spermatozoa of smokers would be more at risk of having increased DNA fragmentation than spermatozoa of non‐smoking men. Methods A cross‐sectional study was performed on consenting smokers and non‐smokers, consulting in an infertility clinic for routine sperm analysis. The application of a novel TUNEL assay coupled to a vitality marker, LIVE/DEAD®, allowed both DNA fragmentation and viability measurement within spermatozoa of participants to be analyzed by flow cytometry. Results The coupled vitality‐DNA fragmentation analysis revealed that non‐smokers and smokers, respectively presented medians of 3.6% [0.6–36.8] and 3.3% [0.9–9.6] DNA fragmented spermatozoa among the living spermatozoa population (P > 0.05). Conclusion No deleterious effect of smoking on spermatozoa was found in our study. More studies concerning potential mutagenic capacities of cigarette smoke on spermatozoa are necessary. In addition, the coupled vitality‐DNA fragmentation analysis may orient Assisted Reproductive Technology teams when confronted with patients having a high percentage of DNA‐fragmented living spermatozoa. © 2014 International Clinical Cytometry Society
ISSN:1552-4949
1552-4957
DOI:10.1002/cyto.b.21185