Modification of energy balance induced by the food contaminant T-2 toxin: A multimodal gut-to-brain connection

• Published in: Brain, behavior, and immunity behavior, and immunity, 2014-03, Vol.37 (3), p.54-72
• Main Authors: Gaigé, Stéphanie, Djelloul, Mehdi, Tardivel, Catherine, Airault, Coraline, Félix, Bernadette, Jean, André, Lebrun, Bruno, Troadec, Jean-Denis, Dallaporta, Michel
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Animals; Anorexia; Antioxidants - metabolism; Biochemistry, Molecular Biology; Blood Glucose - analysis; Brain - drug effects; Brain - metabolism; c-Fos; Calorimetry; Cellular Biology; Energy Metabolism - drug effects; Feeding Behavior - drug effects; Genomics; Illness Behavior - drug effects; Inflammation; Life Sciences; Liver - metabolism; Male; Mice, Inbred C57BL; Molecular biology; Motor Activity - drug effects; Neurobiology; Neurons and Cognition; Oxidative Stress; Psychiatry; ROS; Sickness behavior; Spleen - metabolism; Subcellular Processes; T-2 Toxin - toxicity; Trichothecenes
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2013.12.008

Abstract T-2 toxin is one of the most toxic Fusarium -derived trichothecenes found on cereals and constitutes a widespread contaminant of agricultural commodities as well as commercial foods. Low doses toxicity is characterized by reduced weight gain. To date, the mechanisms by which this mycotoxin profoundly modifies feeding behavior remain poorly understood and more broadly the effects of T-2 toxin on the central nervous system (CNS) have received limited attention. Through an extensive characterization of sickness-like behavior induced by T-2 toxin, we showed that its per os ( p.o .) administration affects not only feeding behavior but also energy expenditure, glycaemia, body temperature and locomotor activity. Using c-Fos expression mapping, we identified the neuronal structures activated in response to T-2 toxin and observed that the pattern of neuronal populations activated by this toxin resembled that induced by inflammatory signals. Interestingly, part of neuronal pathways activated by the toxin were NUCB-2/nesfatin-1 expressing neurons. Unexpectedly, while T-2 toxin induced a strong peripheral inflammation, the brain exhibited limited inflammatory response at a time point when anorexia was ongoing. Unilateral vagotomy partly reduced T-2 toxin-induced brainstem neuronal activation. On the other hand, intracerebroventricular (icv) T-2 toxin injection resulted in a rapid (