Imaging of dense cell cultures by multiwavelength lens‐free video microscopy

They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficie...

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Bibliographic Details
Published in:Cytometry. Part A 2017-05, Vol.91 (5), p.433-442
Main Authors: Allier, C., Morel, S., Vincent, R., Ghenim, L., Navarro, F., Menneteau, M., Bordy, T., Hervé, L., Cioni, O., Gidrol, X., Usson, Y., Dinten, J.‐M.
Format: Article
Language:English
Subjects:
Biotechnology
Cell Count - methods
Cell culture
Cell Culture Techniques
Cell lines
Cell Movement - genetics
Cytometry
dense cell cultures
Epithelial cells
Epithelial Cells - cytology
Field of view
Holography - methods
Humans
Illumination
Image contrast
Image processing
Image reconstruction
Image segmentation
Lenses
lens‐free microscopy
Life Sciences
Mesenchyme
Microscopy
Microscopy, Video - methods
Phase shift
quantitative microscopy
video cytometry
Wavelengths
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Summary:They present results for lens‐free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm2) over a large field of view of 29.4 mm2. Combined with the compactness of the system which fits altogether inside an incubator, lens‐free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens‐free video‐microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry
ISSN:1552-4922
1552-4930
DOI:10.1002/cyto.a.23079