A new bioassay for the inspection and identification of TBT-containing antifouling paint

Since the 1960s tributyl (TBT)-based antifouling paints are widely applied to protect ship’s hulls from biofouling. Due to its high toxicity to aquatic ecosystem most of the countries (28 nations in 2008) signed the AFS convention to control the use of harmful antifouling systems on ships. Neverthel...

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Bibliographic Details
Published in:Marine pollution bulletin 2009-11, Vol.58 (11), p.1734-1738
Main Authors: Gueuné, Hervé, Thouand, Gérald, Durand, Marie-José
Format: Article
Language:English
Subjects:
Antifouling
Bacteria
Bioassay
Biological Assay - methods
Bioluminescence
Control systems
Detection
Environmental Monitoring - instrumentation
Environmental Sciences
Escherichia coli - metabolism
Inspection
Luminescent Proteins - metabolism
Organotin
Organotin Compounds - analysis
Paint
Paint - analysis
Paints
Protective coatings
Ship hulls
Ships
Trialkyltin Compounds - analysis
Water Pollutants, Chemical - analysis
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Summary:Since the 1960s tributyl (TBT)-based antifouling paints are widely applied to protect ship’s hulls from biofouling. Due to its high toxicity to aquatic ecosystem most of the countries (28 nations in 2008) signed the AFS convention to control the use of harmful antifouling systems on ships. Nevertheless there is currently no simple method to control the presence of organotin in paint. In this study, we propose a bioassay based on the use of a recombinant bioluminescent bacteria to detect directly in paint the presence of TBT. We also propose a simple device as an inspection system to control the absence of organotin in the ship’s hull paint. The presence of organotin could be revealed in less than three hours.
ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2009.09.012