Simultaneous Regulation of Cytokinetic Furrow and Nucleus Positions by Cortical Tension Contributes to Proper DNA Segregation during Late Mitosis

Coordinating mitotic spindle and cytokinetic furrow positioning is essential to ensure proper DNA segregation. Here, we present a novel mechanism, which corrects DNA segregation defects due to cytokinetic furrow mispositioning during the first division of C. elegans embryos. Correction of DNA segreg...

Full description

Saved in:
Bibliographic Details
Published in:Current biology 2019-11, Vol.29 (22), p.3766-3777.e4
Main Authors: Pacquelet, Anne, Jousseaume, Matthieu, Etienne, Jocelyn, Michaux, Grégoire
Format: Article
Language:English
Subjects:
Cellular Biology
cortical blebbing
cortical tension
cytokinesis
cytoplasmic flow
Development Biology
furrow positioning
Life Sciences
myosin
Subcellular Processes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Coordinating mitotic spindle and cytokinetic furrow positioning is essential to ensure proper DNA segregation. Here, we present a novel mechanism, which corrects DNA segregation defects due to cytokinetic furrow mispositioning during the first division of C. elegans embryos. Correction of DNA segregation defects due to an abnormally anterior cytokinetic furrow relies on the concomitant and opposite displacements of the furrow and of the anterior nucleus toward the posterior and anterior poles of the embryo, respectively. It also coincides with cortical blebbing and an anteriorly directed cytoplasmic flow. Although microtubules contribute to nuclear displacement, relaxation of an excessive tension at the anterior cortex plays a central role in the correction process and simultaneously regulates cytoplasmic flow as well as nuclear and furrow displacements. This work thus reveals the existence of a so-far uncharacterized correction mechanism, which is critical to correct DNA segregation defects due to cytokinetic furrow mispositioning. [Display omitted] •DNA segregation defects due to furrow mispositioning can be corrected•Correction involves opposite nuclear and furrow movements•Cortical relaxation and cytoplasmic flow due to blebbing reposition the furrow•Microtubules and cytoplasmic flow both contribute to nuclear movement Pacquelet et al. describe a mechanism that corrects DNA segregation defects due to furrow mispositioning during the first division of C. elegans embryos. Through their effect on cortical relaxation and cytoplasmic flow, cortical blebs play a key role in the regulation of furrow and nuclear positions, thereby ensuring proper DNA segregation.
ISSN:0960-9822
1879-0445
DOI:10.1016/j.cub.2019.09.013