Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation

[Display omitted] Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance a...

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Bibliographic Details
Published in:International journal of pharmaceutics 2020-01, Vol.574, p.118872-118872, Article 118872
Main Authors: Borowski, Rafael Gomes Von, Barros, Muriel Primon, da Silva, Denise Brentan, Lopes, Norberto Peporine, Zimmer, Karine Rigon, Staats, Charley Christian, de Oliveira, Cristiane Bernardes, Giudice, Emmanuel, Gillet, Reynald, Macedo, Alexandre José, Gnoatto, Simone Cristina Baggio, Zimmer, Aline Rigon
Format: Article
Language:English
Subjects:
Antiadhesive
Antibiofilm
Bacteriology
Biofilm
Capsicum
Coating
Life Sciences
Microbiology and Parasitology
Peptide
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Summary:[Display omitted] Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography-tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2019.118872