Extraction of DNA from soil

There is an increased interest in the extraction of nucleic acids from various environmental samples, since molecular techniques allow less biased access to a greater portion of uncultivable microorganisms. Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbi...

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Bibliographic Details
Published in:European journal of soil biology 2003-10, Vol.39 (4), p.183-190
Main Authors: Robe, Patrick, Nalin, Renaud, Capellano, Carmela, Vogel, Timothy M., Simonet, Pascal
Format: Article
Language:English
Subjects:
ADN
Agronomy. Soil science and plant productions
BACTERIA
Biochemistry and biology
Biological and medical sciences
BIOLOGIE MOLÉCULAIRE
BIOLOGÍA MOLECULAR
Chemical, physicochemical, biochemical and biological properties
Direct lysis
DNA
DNA extraction
EXTRACCIÓN
EXTRACTION
Fundamental and applied biological sciences. Psychology
IDENTIFICACIÓN
IDENTIFICATION
Indirect extraction
Life Sciences
Metagenome
METHODS
MICRO-ORGANISME DU SOL
MICROORGANISMOS DEL SUELO
MOLECULAR BIOLOGY
MÉTHODE
MÉTODOS
Physics, chemistry, biochemistry and biology of agricultural and forest soils
SOIL MICROORGANISMS
Soil science
Soils
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Summary:There is an increased interest in the extraction of nucleic acids from various environmental samples, since molecular techniques allow less biased access to a greater portion of uncultivable microorganisms. Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbiased representation of the microbial diversity. The first approach consists of the direct extraction of nucleic acids from soil through in situ cell lysis followed by DNA purification. The alternative approach is based on the separation of bacteria from the soil particles followed by cell lysis and then DNA purification. Several published methods describe the recovery of highly purified nucleic acids that are well-suited for molecular purposes even though a new challenge concerns the recovery of large bacterial DNAs essential for functional investigation of gene clusters and biosynthetic pathways. This review presents an overview of the available methods to achieve this challenging objective.
ISSN:1164-5563
1778-3615
DOI:10.1016/S1164-5563(03)00033-5