Distribution of Gymnostoma spp. microsymbiotic Frankia strains in New Caledonia is related to soil type and to host-plant species

The diversity of the Frankia strains that are naturally in symbiosis with plants belonging to the Gymnostoma genus in New Caledonia was investigated. A direct molecular characterization of DNA extracted from nodules was performed, followed by characterization by restriction fragment length polymorph...

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Bibliographic Details
Published in:Molecular ecology 1999-11, Vol.8 (11), p.1781-1788
Main Authors: Navarro, E, Jaffre, T, Gauthier, D, Gourbiere, F, Rinaudo, G, Simonet, P, Normand, P
Format: Article
Language:English
Subjects:
acid soils
acidic alluvium
alluvium
brown hypermagnesian inceptisols
Casuarinaceae
diversity
Frankia
genetic variation
Gymnostoma
host plants
Inceptisols
intergenic DNA
Life Sciences
New Caledonia
nickel
Oxisols
restriction fragment length polymorphism
ribosomal DNA
root nodules
serpentine soils
strains
symbiosis
ultramafic alluvium
ultramafic soil
ultramafic soils
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Summary:The diversity of the Frankia strains that are naturally in symbiosis with plants belonging to the Gymnostoma genus in New Caledonia was investigated. A direct molecular characterization of DNA extracted from nodules was performed, followed by characterization by restriction fragment length polymorphism (RFLP) of the ribosomal rrs–rrl (16S–23S) intergenic spacer (IGS) polymerase chain reaction (PCR)‐amplified region. Seventeen different patterns were identified among the 358 microsymbiotic strains studied in the eight species of host plant present in New Caledonia. This genotypical approach permitted us to show that a large diversity existed among the patterns and that these did not exhibit a strict specificity to any host‐plant species comparable with that previously found in the Casuarina and Allocasuarina symbioses in Australia. Despite this lack of specificity, a correspondence analysis nevertheless showed that the distribution of these patterns was related to soil type and to host‐plant species. Furthermore, several Frankia strains were exclusively associated with the ultramafic soils.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294x.1999.00742.x