Endogenous isoflavone methylation correlates with the in vitro rooting phases of Spartium junceum L. (Leguminosae)

Spartium junceum L. (Leguminosae) is a perennial shrub, native to the Mediterranean region in southern Europe, widespread in all the Italian regions and, as a leguminous species, it has a high isoflavone content. An in vitro culture protocol was developed for this species starting from stem nodal se...

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Bibliographic Details
Published in:Journal of plant physiology 2014-09, Vol.171 (14), p.1267-1275
Main Authors: Clematis, Francesca, Viglione, Serena, Beruto, Margherita, Lanzotti, Virginia, Dolci, Paola, Poncet, Christine, Curir, Paolo
Format: Article
Language:English
Subjects:
4′,5,7-Trimethoxyisoflavone
Chromatography, High Pressure Liquid
Correlation
Environmental Sciences
High performance liquid chromatography
HPLC
In vitro rooting
In vitro testing
Isoflavone methylation
Isoflavones
Isoflavones - metabolism
Life Sciences
Methylation
Phases
Physiology
Plant Roots - growth & development
Plant Roots - metabolism
Roots
Spartium - genetics
Spartium - growth & development
Spartium - metabolism
Spartium junceum
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Summary:Spartium junceum L. (Leguminosae) is a perennial shrub, native to the Mediterranean region in southern Europe, widespread in all the Italian regions and, as a leguminous species, it has a high isoflavone content. An in vitro culture protocol was developed for this species starting from stem nodal sections of in vivo plants, and isoflavone components of the in vitro cultured tissues were studied by means of High Performance Liquid Chromatography (HPLC) analytical techniques. Two main isoflavones were detected in the S. junceum tissues during the in vitro propagation phases: Genistein (4′,5,7-Trihydroxyisoflavone), already reported in this species, and its methylated form 4′,5,7-Trimethoxyisoflavone, detected for the first time in this plant species (0.750±0.02mgg−1 dry tissue). The presence of both of these compounds in S. junceum tissues was consistently detected during the in vitro multiplication phase. The absence of the methylated form within plant tissues in the early phases of the in vitro adventitious root formation was correlated with its negative effect displayed on root induction and initiation phases, while its presence in the final “root manifestation” phase influenced positively the rooting process. The unmethylated form, although detectable in tissues in the precocious rooting phases, was no longer present in the final rooting phase. Its effect on rooting, however, proved always to be beneficial.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2014.03.013