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Neutral sugar side chains of pectins limit interactions with procyanidins

•Pectic hairy regions–procyanidins interactions has been studied by ITC.•The higher the pectic ramifications, the lower association with procyanidins is.•Structures of both compounds are implicated. Interactions between seven hairy regions of pectins, rhamnogalacturonans II and arabinogalactan–prote...

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Bibliographic Details
Published in:Carbohydrate polymers 2014, Vol.99, p.527-536
Main Authors: Watrelot, Aude A., Le Bourvellec, Carine, Imberty, Anne, Renard, Catherine M.G.C.
Format: Article
Language:English
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Summary:•Pectic hairy regions–procyanidins interactions has been studied by ITC.•The higher the pectic ramifications, the lower association with procyanidins is.•Structures of both compounds are implicated. Interactions between seven hairy regions of pectins, rhamnogalacturonans II and arabinogalactan–proteins and procyanidins with different average degrees of polymerization, low (DP9) and high (DP30), were investigated by isothermal titration calorimetry and absorption analysis to study the impact of neutral sugar side chains of pectins on these associations. Associations between pectic fractions and procyanidins involved hydrophobic interactions and hydrogen bonds. No difference in association constants between various hairy regions and procyanidins DP9 was found. Nevertheless, arabinan chains showed lower association constants, and hairy regions of pectins with only monomeric side chains showed higher association with procyanidin DP30. Only very low affinities were obtained with rhamnogalacturonans II and arabinogalactan–proteins. Aggregation could be observed only with the procyanidins of DP30 and the protein-rich arabinogalactan–protein. Associations were obtained at both degrees of polymerization of the procyanidins, but differed depending on neutral sugar composition and the structure of pectic fractions.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2013.08.094