New Process for Fungal Delignification of Sugar-Cane Bagasse and Simultaneous Production of Laccase in a Vapor Phase Bioreactor

We propose a new process using a vapor phase bioreactor (VPB) to simultaneously (i) delignify sugar-cane bagasse, a residue of sugar production that can be recycled in paper industry, and (ii) produce laccase, an enzyme usable to bleach paper pulp. Ethanol vapor, used as laccase inducer, was blown u...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-05, Vol.54 (11), p.3852-3858
Main Authors: Meza, Juan Carlos, Sigoillot, Jean-Claude, Lomascolo, Anne, Navarro, David, Auria, Richard
Format: Article
Language:English
Subjects:
biobleaching
Biological and medical sciences
Bioreactors
biosynthesis
Biotechnology
delignification
ethanol
Ethanol - pharmacology
ethanol vapor phase bioreactors
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
industrial microbiology
Industry
Kinetics
laccase
Laccase - biosynthesis
Laccase - metabolism
Life Sciences
Lignin - metabolism
Methods. Procedures. Technologies
Phenols - analysis
Polyporaceae - enzymology
Polyporaceae - metabolism
pulp
pulping
Pycnoporus
Pycnoporus cinnabarinus
Saccharum - metabolism
Saccharum - microbiology
Sugar industries
sugarcane bagasse
vapors
Various methods and equipments
Volatilization
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Summary:We propose a new process using a vapor phase bioreactor (VPB) to simultaneously (i) delignify sugar-cane bagasse, a residue of sugar production that can be recycled in paper industry, and (ii) produce laccase, an enzyme usable to bleach paper pulp. Ethanol vapor, used as laccase inducer, was blown up through a VPB packed with bagasse and inoculated with Pycnoporus cinnabarinus ss3, a laccase-hyperproducing fungal strain. After 28 days, the laccase activity in the ethanol-treated bagasse was 80-fold higher (80 U gds -1) and the bagasse delignification percentage was 12-fold (12%) higher than in the reference samples produced in the absence of ethanol, corresponding to a high overall pulp yield of 96.1%. In the presence of ethanol, the total soluble phenols amount was 2.5-fold (3 mg FA gds -1) higher than that without ethanol. Six monomeric phenols were detected:  p-coumaric (4-hydroxyphenyl-2-propenoic), ferulic (4-hydroxy-3-methoxyphenyl-2-propenoic), syringic (4-hydroxy-3,5-dimethoxybenzoic), vanillic (4-hydroxy-3-methoxybenzoic) and 4-hydroxybenzoic acids, and 2-methoxyhydroquinone. Higher concentrations of phenolic compounds were observed when ethanol vapor was added, confirming a more efficient bagasse delignification. After 28 days, the fungal-treated bagasse (with ethanol addition) was pulped and refined. For a freeness of 81 mL CSF, this processing required 50% less energy than with untreated bagasse (without inoculation and ethanol addition), which indicated a significant potential economy for the pulp and paper industry. Handsheets were made from pulp obtained after fungal-treated and untreated bagasse. Comparison of bagasse-pulp characteristics for freeness of 35 and 181 mL CSF showed an average increment by 35% for tensile index and breaking strength and length. VPB allowed a simultaneous production of laccase (90 U gds -1, after pressing of the bagasse) that improved the overall profitability of the process. Keywords: Ethanol vapor; bagasse; Pycnoporus cinnabarinus; laccase; delignification; mechanical pulp
ISSN:0021-8561
1520-5118
DOI:10.1021/jf053057j