Interactions between apple ( Malus x domestica Borkh.) polyphenols and cell walls modulate the extractability of polysaccharides

• Published in: Carbohydrate polymers 2009-01, Vol.75 (2), p.251-261
• Main Authors: Le Bourvellec, C., Guyot, S., Renard, C.M.G.C.
• Format: Article
• Language: English
• Subjects: Applied sciences; Biological and medical sciences; Chemical Sciences; Exact sciences and technology; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Hemicellulose; Malus; Natural polymers; Pectin; Physicochemistry of polymers; Polymers; Sequential extraction; Starch and polysaccharides; Tannin
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2008.07.010

Apple polyphenol (procyanidin)–cell wall interactions were investigated and their impact on polysaccharide extractability were determined. Native and oxidised procyanidins with average degrees of polymerisation of 13 and 55 were incubated with cell walls. The effect of polyphenol oxidation was evaluated according to two designs: polyphenols were chemically oxidised either before or during interaction. The extent of procyanidin binding to cell walls was assessed by the weight increase of procyanidin–cell wall complexes as compared to weights of cell walls alone. Pectins and hemicelluloses were subsequently extracted from cell walls and from cell wall–procyanidin adducts using a chelating agent (ammonium oxalate), a pectin lyase treatment and NaOH. Weight increases of complexes ranged from 20% to 29%. Weight gains increased in the following order: native, pre-oxidised, simultaneously oxidised and bound procyanidins, these different fractions were, respectively, bound to cell walls. In presence of native procyanidins, oxalate extracted less pectins, and those pectins had lower degrees of methylation, as compared to cell walls alone. When cell walls were incubated with oxidised and oxidising procyanidins, even less pectins with lower degree of methylation were extracted. Major findings indicated that procyanidins mainly bound to pectins as compared to other cell wall compounds: (1) the procyanidin adsorption to cell walls limited the depolymerisation of pectins supposedly induced by pectin lyase. Thus less pectins were extracted but their degree of methylation increased, indicative of products of lysis of pectin lyase. (2) Hemicelluloses extracted using NaOH (4 M) were more abundant in pectins when oxidised or oxidising procyanidins were complexed rather than non complexed to cell walls.