Spatial arrangement of pig MHC class I sequences

Bacterial artificial chromosome (BAC) clones were assigned within the pig major histocompatibility complex (Mhc) by polymerase chain reaction-screening and Southern blot hybridization using sequence-tagged site (STS) markers and BAC end-rescued sequences. In all, 35 BAC clones were discovered contai...

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Bibliographic Details
Published in:Immunogenetics (New York) 1999-10, Vol.49 (11-12), p.919-930
Main Authors: Velten, F W, Renard, C, Rogel-Gaillard, C, Vaiman, M, Chardon, P
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blotting, Southern
Cells, Cultured
Chromosomes, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Fibroblasts - metabolism
Gene Library
Genes, MHC Class I
Genetic Vectors - genetics
Graft Rejection - prevention & control
Haplotypes - genetics
Humans
Life Sciences
Major Histocompatibility Complex - genetics
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Sequence Alignment
Sequence Homology, Nucleic Acid
Swine - genetics
Swine - immunology
Swine, Miniature - genetics
Transcription, Genetic
Transplantation, Heterologous - immunology
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Summary:Bacterial artificial chromosome (BAC) clones were assigned within the pig major histocompatibility complex (Mhc) by polymerase chain reaction-screening and Southern blot hybridization using sequence-tagged site (STS) markers and BAC end-rescued sequences. In all, 35 BAC clones were discovered containing 12 anchor genes of the SLA class I region and two genes of the SLA class III region. Twenty of these 35 clones comprised two distinct class I gene clusters, each spanning about 100 kilobases. One cluster enclosed three class I related genes (SLA-6 to -8) and two genes (MIC-1 and MIC-2) more distantly related to class I. The other cluster enclosed typical class I genes, of which three (SLA-1, -2, and -3) were transcribed by fibroblasts homozygous for the H01 haplotype which we used to construct a pig BAC library. Ordered clones are certainly helpful in isolating agronomically, biologically, and medically important genes. They would also be useful for inducing genetic modifications in pig cell lines.
ISSN:0093-7711
1432-1211
DOI:10.1007/s002510050575