Effects of endogenous ABA levels and temperature on cedar (Cedrus libani Loudon) bud dormancy in vitro

Axillary and apical buds of in-vitro-propagated cuttings of Cedrus libani are unable to burst at 24 degrees C, but this inhibition was overcome at 30 degrees C. Here we have used cedar microcuttings to investigate whether the levels of endogenous hormones vary with bud dormancy and temperature. We a...

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Bibliographic Details
Published in:Plant cell reports 1998-12, Vol.18 (3/4), p.279-283
Main Authors: Piola, F, Label, P, Vergne, P, Von Aderkas, P, Rohr, R
Format: Article
Language:English
Subjects:
abscisic acid
Acetic acid
Agronomy. Soil science and plant productions
Biological and medical sciences
buds
Cedrus libani
chemical constituents of plants
clones
conifer needles
dormancy breaking
dose response
Economic plant physiology
esters
Fractionation
Fundamental and applied biological sciences. Psychology
genetic variation
Genetics
glucose
Growth and development
Hormones
in vitro culture
indole acetic acid
isopentenyladenine
isopentenyladenosine
Life Sciences
Liquid chromatography
Metabolites
methodology
micropropagation
Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence
Plant growth. Development of the storage organs
Plant physiology and development
Plants genetics
quantitative analysis
regulation
stems
temperature
Vegetative apparatus, growth and morphogenesis. Senescence
zeatin
zeatin riboside
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Summary:Axillary and apical buds of in-vitro-propagated cuttings of Cedrus libani are unable to burst at 24 degrees C, but this inhibition was overcome at 30 degrees C. Here we have used cedar microcuttings to investigate whether the levels of endogenous hormones vary with bud dormancy and temperature. We analysed the levels of abscisic acid, indole-3-acetic acid, zeatin, isopentenyladenine and their major metabolites using HPLC purification and fractionation of the samples coupled to an ELISA method for hormonal quantitation involving several antibodies elicited against each hormonal family. Abscisic acid levels in microcuttings with dormant buds were higher than those in microcuttings with growing buds. At 24 degrees C, needles accumulated more abscisic acid than at 30 degrees C. In addition, when needles were removed, but growth release was achieved at 24 degrees C. Abscisic acid supplied at 30 degrees C induced the formation of dormant buds. These results suggest that abscisic acid accumulation in the needles can explain the bud dormancy of cedar microcuttings at 24 degrees C.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050571