Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal antibodies

The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-14...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-07, Vol.99 (3), p.1099-1104
Main Authors: Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium), Thibault, J.F, Cutsem, P. van
Format: Article
Language:English
Subjects:
Antibodies
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
AZUCARES ACIDOS
Biological and medical sciences
Calcium
Cell biochemistry
Cell physiology
Cellular and Structural Biology
Centrifugation
Dimers
Enzyme linked immunosorbent assay
Epitopes
ESTERIFICACION
ESTERIFICATION
FISIOLOGIA VEGETAL
Fundamental and applied biological sciences. Psychology
Genetics
Life Sciences
Magnesium
Monoclonal antibodies
MORFOGENESIS
MORPHOGENESE
Oligomers
PECTINAS
PECTINE
PHYSIOLOGIE VEGETALE
Plant physiology and development
Plants
Plants genetics
POLIMERIZACION
POLYMERISATION
SUCRE ACIDE
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Summary:The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-1424) has been tested by enzyme-linked immunosorbent assays. Oligomers of DP9 and above preincubated with the antibodies clearly inhibited the association between the antibodies and immobilized pectin. A minimum DP of nine consecutive galacturonic residues is thus necessary to be associated through calcium cations to form dimers. Randomly deesterified pectin was recognized by the antibody if its degree of methylesterification was 30%, whereas blockwise deesterified pectin was recognized up to 40% of methylesterification. The replacement of calcium ions by magnesium prevented the recognition of polygalacturonic acid by the antibody
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.99.3.1099