Development and characterization of a PLGA-HA composite material to fabricate 3D-printed scaffolds for bone tissue engineering

• Published in: Materials Science & Engineering C 2021-01, Vol.118, p.111334-111334, Article 111334
• Main Authors: Babilotte, Joanna, Martin, Benoit, Guduric, Vera, Bareille, Reine, Agniel, Rémy, Roques, Samantha, Héroguez, Valérie, Dussauze, Marc, Gaudon, Manuel, Le Nihouannen, Damien, Catros, Sylvain
• Format: Article
• Language: English
• Subjects: Additive manufacturing; Animals; Biocompatibility; Biocompatible Materials; Biodegradation; Biomaterials; Biomedical materials; Bone and Bones; Bone tissue engineering; Bones; Cell proliferation; Cell viability; Chemical Sciences; Composite; Composite materials; Durapatite; Fabrication; Fused deposition modeling; Humans; Hydroxyapatite; Inflammation; Material chemistry; Materials science; Mechanical loading; Nanoparticles; Physicochemical properties; Polylactide-co-glycolide; Polymer; Printing, Three-Dimensional; Rapid manufacturing; Rats; Reproducibility of Results; Scaffolds; Stress concentration; Surgical implants; Three dimensional composites; Three dimensional models; Three dimensional printing; Tissue Engineering; Tissue Scaffolds
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2020.111334

Additive manufacturing is a rising field in bone tissue engineering. Additive fabrication offers reproducibility, high precision and rapid manufacture of custom patient-specific scaffolds. The development of appropriate composite materials for biomedical applications is critical to reach clinical application of these novel biomaterials. In this work, medical grade poly(lactic-co-glycolic) acid (PLGA) was mixed with hydroxyapatite nanoparticles (nHA) to fabricate 3D porous scaffolds by Fused Deposition Modeling. We have first confirmed that the composite material could be printed in a reproductive manner. Physical characterization demonstrated a low degradation of the material during manufacturing steps and an expected loading and homogeneous distribution of nHA. In vitro biodegradation of the scaffolds showed modifications of morphological and physicochemical properties over time. The composite scaffolds were biocompatible and high cell viability was observed in vitro, as well as a maintain of cell proliferation. As expected, the addition of nHA displayed a positive impact on osteodifferentiation in vitro. Furthermore, a limited inflammatory reaction was observed after subcutaneous implantation of the materials in the rat. Overall, this study suggests that this composite material is suitable for bone tissue engineering applications. •Manufacturing steps have a minor impact on material properties•Materials provide a biocompatible and osteopromotive support over two cell types•No major signals of inflammation observed, outlining material biocompatibility