High through-put characterization of insect morphocryptic entities by a non-invasive method using direct-PCR of fecal DNA

The development of non-invasive molecular techniques is currently increasing, particularly in the fields of behavioural ecology and conservation genetics of mammals. Surprisingly, genetic studies of Arthropods and particularly the insects have not benefited yet from the contributions that non-invasi...

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Bibliographic Details
Published in:Journal of biotechnology 2005-09, Vol.119 (1), p.15-19
Main Authors: Fumanal, B., Martin, J.-F., Bon, M.C.
Format: Article
Language:English
Subjects:
Animals
Arthropoda
Base Sequence
Biochemistry, Molecular Biology
Biological and medical sciences
Biotechnology
Ceutorhynchus assimilis
Coleoptera
Curculionidae
DNA - analysis
DNA - genetics
DSCP
Feces
Fundamental and applied biological sciences. Psychology
Genetics, Population
Insect fecal DNA
Insecta
Life Sciences
Molecular biology
Molecular Sequence Data
Non-invasive method
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Weevils - genetics
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Summary:The development of non-invasive molecular techniques is currently increasing, particularly in the fields of behavioural ecology and conservation genetics of mammals. Surprisingly, genetic studies of Arthropods and particularly the insects have not benefited yet from the contributions that non-invasive methods have made. Here, we outline a strategy for identifying phytophagous insect genetic entities based on direct-PCR of fecal DNA combined with double strand conformation polymorphism (DSCP) typing. This allows the differentiation of morphocryptic entities within the species Ceutorhynchus assimilis (Coleoptera: Curculionidae), a candidate biocontrol agent of a noxious weed. The results obtained clearly demonstrate the potential for this method to provide a valuable means for genetic and ecological studies of Arthropods.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2005.04.011