New Haplotypes of Trypanosoma evansi Identified in Dromedary Camels from Algeria

Purpose Surra is a zoonotic disease caused by Trypanosoma evansi ( Trypanozoon ), a salivary trypanosome native to Africa which affects a wide range of mammals worldwide and causes mortality and significant economic loss. The present study was devoted to the molecular characterization of T. evansi d...

Full description

Saved in:
Bibliographic Details
Published in:Acta parasitologica 2021-03, Vol.66 (1), p.294-302
Main Authors: Boutellis, Amina, Bellabidi, Meriem, Benaissa, Mohammed Hocine, Harrat, Zoubir, Brahmi, Karima, Drali, Rezak, Kernif, Tahar
Format: Article
Language:English
Subjects:
Animal biology
Animal Systematics/Taxonomy/Biogeography
Biochemistry, Molecular Biology
Biomedical and Life Sciences
Biomedicine
Camelidae
Camels
Deoxyribonucleic acid
DNA
DNA sequencing
Ecology
Economic impact
Epidemiology
Genetic diversity
Geographical locations
Haplotypes
Life Sciences
Medical Microbiology
Microbiology
Microbiology and Parasitology
Molecular biology
Nucleotide sequence
Original Paper
Parasitology
Surra
Trypanosoma
Trypanosoma evansi
Trypanosome
Veterinary medicine and animal Health
Zoonoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose Surra is a zoonotic disease caused by Trypanosoma evansi ( Trypanozoon ), a salivary trypanosome native to Africa which affects a wide range of mammals worldwide and causes mortality and significant economic loss. The present study was devoted to the molecular characterization of T. evansi derived from naturally infected dromedary camels in Algeria. Methods A total of 148 blood samples were collected from mixed age camels living in one of four geographic regions (Ouargla, El Oued, Biskra and Ghardaia) of Algeria. Samples underwent PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) complete sequence. Results DNA of Trypanosoma spp. was found in 19 camels (12.84%). Trypanosoma spp. molecular positivity was not affected by sex ( p  = 0.50), age ( p  = 0.08), or geographic location ( p  = 0.12). Based on multiple sequence alignment of the obtained DNA sequences with representative T. evansi ITS1 sequences available globally, the Algerian sequences were grouped within four different haplotypes including two which were original. Conclusion Results of this study provide preliminary data on which future studies of genetic diversity and molecular epidemiology of T. evansi can be based.
ISSN:1230-2821
1896-1851
DOI:10.1007/s11686-020-00316-w