MRSI vs CEST MRI to understand tomato metabolism in ripening fruit: is there a better contrast?

Besides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better understand fruit development and metabolism. Routine methods based on fixation f...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2021-02, Vol.413 (5), p.1251-1257
Main Authors: Pagés, Guilhem, Deborde, Catherine, Lemaire-Chamley, Martine, Moing, Annick, Bonny, Jean-Marie
Format: Article
Language:English
Subjects:
Amino acids
Analytical Chemistry
Biochemistry
Carbohydrates
Characterization and Evaluation of Materials
Chemical equilibrium
Chemistry
Chemistry and Materials Science
Communication
Comparative analysis
Food Science
Fruits
Glutamine
Laboratory Medicine
Life Sciences
Magnetic resonance imaging
Magnetic resonance spectroscopy
Mapping
Medical imaging
Metabolism
Metabolites
Methods
Monitoring/Environmental Analysis
NMR
Nuclear magnetic resonance
Nuclear magnetic resonance spectroscopy
Observations
Organs
Physiological aspects
Protons
Ripening
Tomatoes
Vegetal Biology
γ-Aminobutyric acid
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Summary:Besides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better understand fruit development and metabolism. Routine methods based on fixation for cytological studies or dissection for metabolite measurements induce biases and plant sample destruction. Magnetic resonance spectroscopy imaging (MSRI) leads to one NMR spectrum per pixel while chemical exchange saturation transfer (CEST) MRI allows mapping metabolites having exchangeable protons. As both methods present different advantages and drawbacks, we compared them to map metabolites in ripe tomato fruits. We demonstrated that MRSI was difficult to interpret due to large spatial chemical shift variations while CEST MRI produced promising image mapping of the main carbohydrates and amino acids. It showed that glucose/fructose was mostly located in the locular tissue, whereas glutamate/glutamine/GABA was found inside the columella. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-03101-w