Enzymatic depolymerization of industrial lignins by laccase‐mediator systems in 1,4‐dioxane/water

Lignin is the second most abundant polymer after cellulose in lignocellulosic biomass. Its aromatic composition and recalcitrant nature make its valorization a major challenge for obtaining low molecular weight aromatics compounds with high value‐added from the enzymatic depolymerization of industri...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and applied biochemistry 2020-09, Vol.67 (5), p.774-782
Main Authors: Dillies, Justine, Vivien, Céline, Chevalier, Mickael, Rulence, Alexandre, Châtaigné, Gabrielle, Flahaut, Christophe, Senez, Vincent, Froidevaux, Renato
Format: Article
Language:English
Subjects:
Aromatic compounds
Biocatalysis
Cellulose
depolymerisation
Depolymerization
Dioxanes - metabolism
Engineering Sciences
Kraft lignin
Laccase
Laccase - metabolism
Lignin
Lignin - metabolism
Lignocellulose
lignosulfonate
Low molecular weights
mediators
Molecular weight
organosolv lignin
Oxidation
Oxidation-Reduction
Polymers
Polyporaceae - enzymology
Polyporaceae - metabolism
Solubility
Solubilization
Sulfonation
Sulfonic acid
Sulfonic Acids - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lignin is the second most abundant polymer after cellulose in lignocellulosic biomass. Its aromatic composition and recalcitrant nature make its valorization a major challenge for obtaining low molecular weight aromatics compounds with high value‐added from the enzymatic depolymerization of industrial lignins. The oxidation reaction of lignin polymer using laccases alone remains inefficient. Therefore, researches are focused on the use of a laccase‐mediator system (LMS) to facilitate enzymatic depolymerization. Until today, the LMS system was studied using water‐soluble lignin only (commercial lignins, modified lignins, or lignin model compounds). This work reports a study of three LMS systems to depolymerize the three major industrial lignins (organosolv lignin, Kraft lignin, and sodium lignosulfonate). We show that an enzymatic depolymerization of these lignins can be achieved by LMS using laccase from Trametes versicolor, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt as mediator and a cosolvent (25% of 1,4‐dioxane) to enhance the solubilization of lignins. LMS for lignin depolymerization in 1,4‐dioxane/water.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1887