Genome Transplantation in Bacteria: Changing One Species to Another

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycopla...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2007-08, Vol.317 (5838), p.632-638
Main Authors: Lartigue, Carole, Glass, John I, Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P, Hutchison, Clyde A. III, Smith, Hamilton O, Venter, J. Craig
Format: Article
Language:English
Subjects:
Acetate Kinase - chemistry
Acetate Kinase - genetics
Amino Acid Sequence
Bacteria
Bacteriology
Biological and medical sciences
Biotechnology
Cell fusion
Cellular biology
Chromosomes
DNA
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Fundamental and applied biological sciences. Psychology
Gels
Gene transfer
Genetic engineering
Genetic technics
Genetics
Genome, Bacterial
Genomes
Genomics
Genotype
Life Sciences
Methods. Procedures. Technologies
Microbiology
Molecular biology
Molecular Sequence Data
Mycoplasma
Mycoplasma - chemistry
Mycoplasma - genetics
Mycoplasma capricolum
Mycoplasma mycoides
Mycoplasma mycoides - chemistry
Mycoplasma mycoides - genetics
Phenotype
Plasmids
Polyethylene Glycols
Polymerase chain reaction
Proteome - analysis
Recombination, Genetic
Research Article
Sequence Analysis, DNA
Synthetic digonucleotides and genes. Sequencing
Transformation, Bacterial
Transplantation
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Summary:As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1144622