Effects and bioaccumulation of Cr(III), Cr(VI) and their mixture in the freshwater mussel Corbicula fluminea

• Published in: Chemosphere (Oxford) 2022-06, Vol.297, p.134090-134090, Article 134090
• Main Authors: Aharchaou, Imad, Maul, Armand, Pons, Marie-Noëlle, Pauly, Danièle, Poirot, Hélène, Flayac, Justine, Rodius, François, Rousselle, Philippe, Beuret, Maximilien, Battaglia, Eric, Vignati, Davide A.L.
• Format: Article
• Language: English
• Subjects: Animals; Bioaccumulation; Biomarkers; Chemical Sciences; Chromium - analysis; Chromium speciation; Corbicula - metabolism; Ecotoxicology; Environmental Sciences; Fresh Water; Global Changes; Life Sciences; Mixture exposure; Other; Regression analysis; Stable isotopes; Toxicology; Water Pollutants, Chemical - analysis
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2022.134090

Chromium has two main oxidation states, Cr(III) and Cr(VI), that can occur simultaneously in natural waters. Current consensus holds that Cr(VI) is of high ecotoxicological concern, but regards Cr(III) as poorly bioavailable and relatively non-toxic. In this work, the effects and bioaccumulation of Cr(III), Cr(VI) and their mixture were studied using the freshwater clam Corbicula fluminea as a model organism. Mixture exposures were carried out using solutions isotopically enriched in 50Cr(III) or 53Cr(VI), allowing to quantify the contribution of each redox form to total Cr accumulation in the clams. Following exposure to individual redox forms, Cr(III) accumulated preferentially in the digestive glands and Cr(VI) in the gills of C. fluminea. In mixture exposures, both redox forms accumulated mainly in the gills; the concentration of Cr(III) in the digestive glands being much lowered compared with individual exposures. Both oxidation states affected the expression of biomarkers related to energy reserves, cellular damage and mitochondrial functioning, as well as the expression of mRNA for detoxification genes. The observed effects differed between gills and digestive glands. The present study suggests that Cr(III) is a bioavailable and biologically active elemental species deserving more consideration by the ecotoxicological community. [Display omitted] •Biomarkers expression responds to Cr redox state, concentration and exposure duration.•First use of isotopically enriched solutions to study Cr(III)/Cr(VI) mixtures.•Both Cr(VI) and Cr(III) accumulate in gills and digestive glands of Corbicula fluminea.•The presence of Cr(VI) changes the accumulation pattern of Cr(III).•Cr(III) is a biologically active species along with Cr(VI).