Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor

Deep fluorescence imaging in mammalian brain tissues remains challenging due to scattering and optical aberration-induced loss in signal and resolution. Correction of aberrations using adaptive optics (AO) requires their reliable measurement in the tissues. Here, we show that an extended-source Shac...

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Bibliographic Details
Published in:Optics express 2022-04, Vol.30 (9), p.15250-15265
Main Authors: Imperato, Sophia, Harms, Fabrice, Hubert, Antoine, Mercier, Mathias, Bourdieu, Laurent, Fragola, Alexandra
Format: Article
Language:English
Subjects:
Animals
Brain - diagnostic imaging
Mammals
Mice
Optical Imaging
Optics
Optics and Photonics
Physics
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Summary:Deep fluorescence imaging in mammalian brain tissues remains challenging due to scattering and optical aberration-induced loss in signal and resolution. Correction of aberrations using adaptive optics (AO) requires their reliable measurement in the tissues. Here, we show that an extended-source Shack-Hartmann wavefront sensor (ESSH) allows quantitative aberration measurements through fixed brain slices with a thickness up to four times their scattering length. We demonstrate in particular that this wavefront measurement method based on image correlation is more robust to scattering compared to the standard centroid-based approach. Finally, we obtain a measurement of the tissue scattering length taking advantage of the geometry of a Shack-Hartmann sensor.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.456651