Identification of a common epitope in knottins and phospholipases D present in Loxosceles sp venom by a monoclonal antibody

In the Americas and specially in Brazil, the Loxosceles intermedia, Loxosceles gaucho and Loxosceles laeta are the three most medically relevant brown spider species, and whose bites can lead to the condition known as loxoscelism. Here, we report the development of a tool capable of identifying a co...

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Bibliographic Details
Published in:International journal of biological macromolecules 2023-08, Vol.246, p.125588-125588, Article 125588
Main Authors: Alves-Mondini, Camila, Beltramino, Martina, Jiacomini, Isabella Gizzi, Karim-Silva, Sabrina, dos Santos Antunes, Nicolle, de Moura, Juliana, Aubrey, Nicolas, Billiald, Philippe, Machado-de-Ávila, Ricardo Andrez, Alvarenga, Larissa M., Becker-Finco, Alessandra
Format: Article
Language:English
Subjects:
Cross-reactivity
Knottins
Life Sciences
Loxosceles
Monoclonal antibodies
Phospholipases D
Recombinant fragments
Synergism
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Summary:In the Americas and specially in Brazil, the Loxosceles intermedia, Loxosceles gaucho and Loxosceles laeta are the three most medically relevant brown spider species, and whose bites can lead to the condition known as loxoscelism. Here, we report the development of a tool capable of identifying a common epitope amongst Loxosceles sp. venom's toxins. A murine monoclonal antibody (LmAb12) and its recombinant fragments (scFv12P and diabody12P) have been produced and characterized. This antibody and its recombinant constructs were able to recognize proteins of Loxosceles spider venoms with specificity. The scFv12P variant was also able to detect low concentrations of Loxosceles venom in a competitive ELISA assay, displaying potential as a venom identification tool. The primary antigenic target of LmAb12 is a knottin, a venom neurotoxin, that has a shared identity of 100 % between the L. intermedia and L. gaucho species and high similarity to L. laeta. Furthermore, we observed LmAb12 was able to partially inhibit in vitro hemolysis, a cellular event typically induced by the Loxosceles sp. venoms. Such behavior might be due to LmAb12 cross-reactivity between the antigenic target of LmAb12 and the venom's dermonecrotic toxins, the PLDs, or even the existence of synergism between these two toxins. [Display omitted]
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.125588