Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

• Published in: Journal of applied microbiology 2020-10, Vol.129 (4), p.1043-1052
• Main Authors: Titouche, Y., Houali, K., Ruiz‐Ripa, L., Vingadassalon, N., NIA, Y., Fatihi, A., Cauquil, A., Bouchez, P., Bouhier, L., Torres, C., Hennekinne, J.A.
• Format: Article
• Language: English
• Subjects: Algeria; Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antimicrobial resistance; antimicrobial sensitivity; Dairy products; Drug resistance; Drug Resistance, Bacterial - genetics; enterotoxin genes; Enterotoxins - genetics; Food; Food Microbiology; Food production; food products; Genes; Genotypes; Health risks; Life Sciences; Meat; Meat products; MecA protein; Methicillin; Methicillin-Resistant Staphylococcus aureus - drug effects; Methicillin-Resistant Staphylococcus aureus - genetics; Methicillin-Resistant Staphylococcus aureus - isolation & purification; methicillin‐resistant S. aureus; Microbial Sensitivity Tests; Multilocus Sequence Typing; Penicillin; Public health; S. aureus; Spas; Staphylococcal enterotoxin G; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus infections
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.14665

Aims The aim of this study was to characterize Staphylococcusaureus isolates of food origin (dairy and meat products, pastries and sandwiches) determining the carriage in enterotoxin genes and the antimicrobial resistance pheno/genotypes. Methods and results A total of 300 food samples were collected and analysed for the detection of S. aureus. The presence of enterotoxin genes was investigated by multiplex PCRs. Resistance of isolates to 11 antimicrobials was determined using disc diffusion method and molecular characterization of methicillin‐resistant S. aureus was carried out by spa typing and multilocus sequence typing. Overall, 51 out of 300 samples (17%) were contaminated with S. aureus, and 104 isolates were recovered. In all, 65 of these isolates (62·5%) harboured one or more genes encoding for staphylococcal enterotoxins, being seg and sei the most observed genes. The highest resistance profile was ascribed to penicillin G (95·19%). Five isolates were methicillin‐resistant (MRSA) harbouring the mecA gene. All MRSA isolates belonged to the sequence type ST5 and to two different spa types (t450 and t688); the MRSA‐t450 isolate carried the scn gene (specific marker of the immune evasion cluster system), but the four MRSA‐t688 isolates were scn negative. The MRSA isolates carried enterotoxin genes but were negative for the genes of the Panton Valentine leukocidine (lukF/S‐PV). Conclusion The presence of enterotoxigenic S. aureus isolates, including MRSA, in food samples can represent a risk for public health. Significance and Impact of this Study This work describes the molecular characteristics of MRSA strains isolated from foods in Algeria and it can contribute to an extended database concerning the S. aureus isolated from food origin.