Selection and optimisation of a method for efficient metabolites extraction from microalgae

[Display omitted] ► Nine disruption techniques were tested on two microalgae models. ► Image analysis was used to evaluate the efficiency of disruption techniques. ► The best grinding method was the mixer mill with polypropylen grinding jars. ► The disruption method was optimised in the objective of...

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Bibliographic Details
Published in:Bioresource technology 2012-11, Vol.124, p.311-320
Main Authors: Serive, Benoît, Kaas, Raymond, Bérard, Jean-Baptiste, Pasquet, Virginie, Picot, Laurent, Cadoret, Jean-Paul
Format: Article
Language:English
Subjects:
Added value
Biofuels
Byproducts
Carbon dioxide
Carbon Dioxide - isolation & purification
Disruption
Extraction
Life Sciences
Metabolites
Microalgae
Microalgae - metabolism
Phaeodactylum tricornutum
Pigment
Polarity
Porphyridium
Porphyridium purpureum
Screening
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Summary:[Display omitted] ► Nine disruption techniques were tested on two microalgae models. ► Image analysis was used to evaluate the efficiency of disruption techniques. ► The best grinding method was the mixer mill with polypropylen grinding jars. ► The disruption method was optimised in the objective of high throughput screening. ► Pigments were good candidates to follow extraction of fragile metabolites. Over the last decade, the use of microalgae for biofuel production and carbon dioxide sequestration has become a challenge worldwide. Processing costs are still too high for these methods to be profitable though, leading to a need to find high value by-products to optimise the added value of this biomass. For high-throughput screening of such metabolites, it is essential to reach the inner content of the cell. This paper presents research and development of a technique enabling a high extraction yield of any metabolite, taking into account the difficulty of extracting bound and or inaccessible molecules with a wide variety of polarities. To this end, several disruption techniques were tested at laboratory scale on two biological models: Porphyridium purpureum and Phaeodactylum tricornutum. A mixer mill gave the best results, offering access to a broad diversity of metabolites from microalgae for high-throughput screening.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.07.105