Efficient Expansion of Human Granzyme B-Expressing B Cells with Potent Regulatory Properties

• Published in: The Journal of immunology (1950) 2020-11, Vol.205 (9), p.2391-2401
• Main Authors: Chesneau, Mélanie, Mai, Hoa Le, Danger, Richard, Le Bot, Sabine, Nguyen, Thi-Van-Ha, Bernard, Josselin, Poullaouec, Cyrielle, Guerrif, Pierrick, Conchon, Sophie, Giral, Magali, Charreau, Béatrice, Degauque, Nicolas, Brouard, Sophie
• Format: Article
• Language: English
• Subjects: Apoptosis; Apoptosis - immunology; B-Lymphocytes, Regulatory; B-Lymphocytes, Regulatory - microbiology; CD40 Ligand; CD40 Ligand - immunology; Cell Differentiation; Cell Differentiation - immunology; Cell Proliferation; Cell Proliferation - physiology; Cells, Cultured; Granzymes; Granzymes - immunology; Humans; Interleukins; Interleukins - immunology; Leukocytes, Mononuclear; Leukocytes, Mononuclear - immunology; Life Sciences
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.2000335

Granzyme B-expressing B cells have been shown to be an important regulatory B cell subset in humans. However, it is unclear which subpopulations of B cells express GZMB under normal conditions and which protocols effectively induce ex vivo expansion of GZMB B cells. We found that in the peripheral blood of normal individuals, plasmablasts were the major B cell subpopulation that expressed GZMB. However, when using an in vitro plasmablast differentiation protocol, we obtained only 2% GZMB B cells. Nevertheless, using an expansion mixture containing IL-21, anti-BCR, CpG oligodeoxynucleotide, CD40L, and IL-2, we were able to obtain more than 90% GZMB B cells after 3 d culture. GZMB B cells obtained through this protocol suppressed the proliferation of autologous and allogenic CD4 CD25 effector T cells. The suppressive effect of GZMB B cells was partially GZMB dependent and totally contact dependent but was not associated with an increase in effector T cell apoptosis or uptake of GZMB by effector T cells. Interestingly, we showed that GZMB produced by B cells promoted GZMB B cell proliferation in ERK1/2-dependent manner, facilitating GZMB B cell expansion. However, GZMB B cells tended to undergo apoptosis after prolonged stimulation, which may be considered a negative feedback mechanism to limit their uncontrolled expansion. Finally, we found that expanded GZMB B cells exhibited a regulatory phenotype and were enriched in CD307b , CD258 CD72 , and CD21loPD-1 B cell subpopulations. Our study, to our knowledge, provides new insight into biology of GZMB B cells and an efficient method to expand GZMB B cells for future cell therapy applications.