Combined Flow‐Fluorescence in situ hybridization to HHV‐8 and EBV reveals the viral heterogeneity of primary effusion lymphoma

Primary effusion lymphoma (PEL) is a rare B‐cell non‐Hodgkin lymphoma associated with Kaposi Sarcoma‐associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein‐Barr virus (EBV) in 60−80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports P...

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Bibliographic Details
Published in:Journal of medical virology 2024-08, Vol.96 (8), p.e29836-n/a
Main Authors: Stammler, Romain, Vacher, Lauriane, Fournier, Benjamin, Lemaire, Pierre, Chauvel, Clémentine, Silvestrini, Marc‐Antoine, Knapp, Silène, Frémont, Grégoire Martin, Meignin, Véronique, Salmona, Maud, Legoff, Jérôme, Vanjak, Anthony, Dunogué, Bertrand, Urbain, Fanny, Lambotte, Olivier, Noël, Nicolas, Gérard, Laurence, Oksenhendler, Eric, Galicier, Lionel, Latour, Sylvain, Boutboul, David
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
CD19 antigen
CD38 antigen
CD40 antigen
Diagnosis
EBV
Effusion
Epstein-Barr Virus Infections - complications
Epstein-Barr Virus Infections - virology
Female
Flow‐Fluorescence in situ hybridization
Fluorescence
Fluorescence in situ hybridization
Herpes viruses
Herpesviridae Infections - diagnosis
Herpesviridae Infections - virology
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - isolation & purification
Herpesvirus 8, Human - genetics
Herpesvirus 8, Human - isolation & purification
Heterogeneity
HHV8
Humans
Hybridization
In Situ Hybridization, Fluorescence - methods
KSHV
Life Sciences
Lymphoma
Lymphoma, Primary Effusion - virology
Male
Middle Aged
Non-Hodgkin's lymphoma
Primary effusion lymphoma
Sarcoma
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Description
Summary:Primary effusion lymphoma (PEL) is a rare B‐cell non‐Hodgkin lymphoma associated with Kaposi Sarcoma‐associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein‐Barr virus (EBV) in 60−80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow‐Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations.
ISSN:0146-6615
1096-9071
1096-9071
DOI:10.1002/jmv.29836