Polymorphonuclear neutrophil response to hydroxyapatite particles, implication in acute inflammatory reaction

Hydroxyapatite (HA) is widely used as a bone substitute or coating biomaterial in bone diseases or prosthesis metal parts. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. Among the hallmarks of such inflammatory response is early recru...

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Bibliographic Details
Published in:Acta biomaterialia 2009-06, Vol.5 (5), p.1708-1715
Main Authors: Velard, Frédéric, Laurent-Maquin, Dominique, Guillaume, Christine, Bouthors, Sylvie, Jallot, Edouard, Nedelec, Jean-Marie, Belaaouaj, Abderrazzaq, Laquerriere, Patrice
Format: Article
Language:English
Subjects:
Bioengineering
Cell Shape - drug effects
Chemical Sciences
Chemotactic Factors - metabolism
Chemotaxis, Leukocyte - drug effects
Cytokines
Cytokines - metabolism
Durapatite - pharmacology
Humans
Hydroxyapatite
Inflammation
Inflammation - enzymology
Inflammation - pathology
Inflammation Mediators - metabolism
Interleukin-8 - secretion
Life Sciences
Material chemistry
Matrix Metalloproteinase 9 - metabolism
Models, Biological
Neutrophil
Neutrophil Activation - drug effects
Neutrophils - cytology
Neutrophils - drug effects
Neutrophils - enzymology
Neutrophils - ultrastructure
Subcellular Fractions - drug effects
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Summary:Hydroxyapatite (HA) is widely used as a bone substitute or coating biomaterial in bone diseases or prosthesis metal parts. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. Among the hallmarks of such inflammatory response is early recruitment of the polymorphonuclear cells (PMNs). The purpose of this work is to investigate the response of PMNs following exposure to HA in terms of secreted mediators. Our study shows that HA particles increase the release of pro-inflammatory mediators such as interleukin-1α, as well as chemotactic factors such as interleukin-8, macrophage inflammatory protein-1α and macrophage inflammatory protein-1β. HA also induces an increase in matrix metalloproteinase 9 expression. Taken together, our data demonstrate for the first time that HA is capable of activating PMNs, a phenomenon that could potentially contribute to the onset of implant-associated inflammation.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2009.01.008