Modulation of benzo[a]pyrene diolepoxide–DNA adduct levels in human white blood cells by CYP1A1, GSTM1 and GSTT1 polymorphism

• Published in: Carcinogenesis (New York) 2000-01, Vol.21 (1), p.35-41
• Main Authors: Rojas, Margarita, Cascorbi, Ingolf, Alexandrov, Kroum, Kriek, Erik, Auburtin, Guy, Mayer, Lucienne, Kopp-Schneider, Annette, Roots, Ivar, Bartsch, Helmut
• Format: Article
• Language: English
• Subjects: (±)-trans-7; 10-epoxy-7; 10-tetrahydrobenzo[a]pyrene; 2A/4; 2B/ 2B; 2B/4; 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - analysis; 8-dihydroxy-anti-9; Adult; B[a]P; benzo[a]pyrene; Biological and medical sciences; BPDE; Chemical and industrial products toxicology. Toxic occupational diseases; CYP1A1; CYP1A11/1; CYP1A11/2 (1/2A; CYP1A12/2 (2/2A; Cytochrome P-450 CYP1A1 - genetics; CYTOCHROME P450 1A1 gene; DNA Adducts - analysis; Environmental Sciences; Genotype; glutathione S-transferase M1 gene; glutathione S-transferase T1 gene; Glutathione Transferase - genetics; GSTM1; GSTT1; heterozygotes; homozygotes; HPLC with fluorometric detection; HPLC-FD; Humans; Isoenzymes - genetics; Lung Neoplasms - etiology; Male; Medical sciences; Multivariate Analysis; number of packs of cigarettes smoked per day multiplied by the number of years of smoking; Occupational Exposure; PAH; PAQ; Pneumology; polycyclic aromatic hydrocarbons; Polymorphism, Genetic; Smoking - adverse effects; Toxicology; Tumors of the respiratory system and mediastinum; Various organic compounds; wild-type
• ISSN: 0143-3334; 1460-2180; 1460-2180
• DOI: 10.1093/carcin/21.1.35

The modulation of benzo[a]pyrene diolepoxide (BPDE)–DNA adduct levels by polymorphisms in the CYP1A1, GSTM1 and GSTT1 genes was assessed in leukocytes of Caucasian males. Eighty-nine coke oven workers (35 smokers, 36 ex-smokers and 18 non-smokers) were recruited from job categories with different exposure levels to polycyclic aromatic hydrocarbons (PAH), together with 44 power plant workers (all smokers) not exposed to PAH. BPDE–DNA adducts were detected in 69 of 133 (52%) DNA samples with a 100-fold variation (range 0.2–44 adducts/108 nt) and a median of 1.6 adducts/108 nt. All samples with the GSTM1 active genotype (n = 59) and five out of 74 samples with GSTM1*0/*0 (7%) showed non-detectable adducts (0.2 adducts/108 nt). The difference in adduct level between the GSTM1*0/*0 and GSTM1 active genotypes was highly significant (P < 0.0001). No significant difference in adduct level between the GSTT1*0/*0 and GSTT1 active genotypes was seen. All heterozygotes (CYP1A1*1/*2) from subjects of GSTM1 active type did not have detectable adducts. Among the GSTM1-deficient individuals (n = 69), 42 with the CYP1A1*1/*1 genotype showed a lower adduct level (median 1.3, range 0.2–4.1 adducts/108 nt) compared with 26 individuals with heterozygous mutated CYP1A1*1/*2 genotypes (median 2.5, range 0.4–6.1 adducts/108 nt, P < 0.015). One individual with low PAH exposure and the rare combination CYP1A1*2A/*2A–GSTM1*0/*0 showed an extremely high level of 44 adducts/108 nt. Significant differences in detectable adduct levels were found between the CYP1A1*1/*1 and CYP1A1*1/*2 genotypes in the exposed group low + medium (P = 0.01) and for all adduct levels, detectable and non-detectable (set at a fixed value), in highly exposed individuals and in ex-smokers (P = 0.03), whereas no such differences were observed in the control group. Mutated CYP1A1*1/*2 increased the adduct level in non-smokers from the exposed group (1.4 versus 2.2 adducts/108 nt), but had no effect on the smokers from the exposed group (2.3 versus 2.8 adducts/108 nt). When all variables were dichotomized, statistical evaluation showed that CYP1A1 status (P = 0.015), PAH exposure (P = 0.003) and smoking (P = 0.006) had significant effects on adduct levels which increased in the order: CYP1A1*1/*1 < CYP1A1(*1/*2 or *2A/*2A); environmental exposure < occupational exposure; non-smokers < smokers, whereby adducts increased with