The in vivo degradation of a ruthenium labelled polysaccharide-based hydrogel for bone tissue engineering

Abstract In this paper we report a new method that permitted for the first time to selectively track a polysaccharide-based hydrogel on bone tissue explants, several weeks after its implantation. The hydrogel, which was developed for bone healing and tissue engineering, was labelled with a ruthenium...

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Bibliographic Details
Published in:Biomaterials 2009-03, Vol.30 (8), p.1568-1577
Main Authors: Laïb, Samia, Fellah, Borhane H, Fatimi, Ahmed, Quillard, Sophie, Vinatier, Claire, Gauthier, Olivier, Janvier, Pascal, Petit, Marc, Bujoli, Bruno, Bohic, Sylvain, Weiss, Pierre
Format: Article
Language:English
Subjects:
Absorbable Implants
Advanced Basic Science
Animals
Biocompatible Materials - metabolism
Bioengineering
Biomaterials
Bone and Bones - drug effects
Bone and Bones - metabolism
Bone healing
Calcium Phosphates - metabolism
Cell Line
Cell Survival - drug effects
Cells, Cultured
Ceramics - metabolism
Chondrocytes - cytology
Chondrocytes - drug effects
Cross-Linking Reagents - pharmacology
Degradation
Dentistry
Femur - pathology
Femur - ultrastructure
Fluorescence
Humans
Hydrogel
Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism
Hypromellose Derivatives
In vivo test
Life Sciences
Methylcellulose - analogs & derivatives
Methylcellulose - chemistry
Methylcellulose - metabolism
N-Acetylneuraminic Acid - chemistry
N-Acetylneuraminic Acid - metabolism
Osteogenesis - drug effects
Prosthesis Implantation
Rabbits
Ruthenium - metabolism
Siloxane
Time Factors
Tissue Engineering
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Summary:Abstract In this paper we report a new method that permitted for the first time to selectively track a polysaccharide-based hydrogel on bone tissue explants, several weeks after its implantation. The hydrogel, which was developed for bone healing and tissue engineering, was labelled with a ruthenium complex and implanted into rabbit bone defects in order to investigate its in vivo degradation. 1, 2, 3 and 8 weeks after surgery, the bone explants were analyzed by synchrotron X-ray microfluorescence, infrared mapping spectroscopy, scanning electron microscopy, and optical microscopy after histological coloration. The results showed that the labelled polysaccharide-based hydrogel was likely to undergo phagocytosis that seemed to occur from the edge to the center of the implantation site up to at least the 8th week.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.11.031