Loading…

Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells‐derived hepatic stem cells

Pluripotent stem cells have been investigated as a renewable source of therapeutic hepatic cells, in order to overcome the lack of transplantable donor hepatocytes. Whereas different studies were able to correct hepatic defects in animal models, they focused on the most mature phenotype of hepatocyt...

Full description

Saved in:
Bibliographic Details
Published in:Xenotransplantation (Københaven) 2020-01, Vol.27 (1), p.e12544-n/a
Main Authors: Fourrier, Angélique, Delbos, Frédéric, Menoret, Séverine, Collet, Camille, Thi Thuy, Linh Trinh, Myara, Anne, Petit, François, Tolosa, Laia, Laplanche, Sophie, Gómez‐Lechón, María José, Labrune, Philippe, Anegon, Ignacio, Vallier, Ludovic, Garnier, Delphine, Nguyen, Tuan Huy
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pluripotent stem cells have been investigated as a renewable source of therapeutic hepatic cells, in order to overcome the lack of transplantable donor hepatocytes. Whereas different studies were able to correct hepatic defects in animal models, they focused on the most mature phenotype of hepatocyte‐like cells (HLCs) derived from pluripotent stem cells and needed freshly prepared cells, which limits clinical applications of HLCs. Here, we report the production of hepatic stem cells (pHSCs) from human‐induced pluripotent stem cells (hiPSCs) in xeno‐free, feeder‐free, and chemically defined conditions using as extracellular matrix a recombinant laminin instead of Matrigel, an undefined animal‐derived matrix. Freshly prepared and frozen pHSCs were transplanted via splenic injection in Gunn rats, the animal model for Crigler‐Najjar syndrome. Following cell transplantation and daily immunosuppression treatment, bilirubinemia was significantly decreased (around 30% decrease, P 
ISSN:0908-665X
1399-3089
DOI:10.1111/xen.12544