Airway cell involvement in intermittent hypoxia-induced airway inflammation

Purpose Respiratory inflammation has been described in patients with obstructive sleep apnea syndrome, but it is unknown whether the increased neutrophil and interleukin (IL)-8 levels observed in induced sputum reflect systemic or local airway inflammation. We assessed the potential role of resident...

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Bibliographic Details
Published in:Sleep & breathing 2015-03, Vol.19 (1), p.297-306
Main Authors: Philippe, C., Boussadia, Y., Prulière-Escabasse, V., Papon, J F., Clérici, C., Isabey, D., Coste, A., Escudier, E., d’Ortho, M P.
Format: Article
Language:English
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Summary:Purpose Respiratory inflammation has been described in patients with obstructive sleep apnea syndrome, but it is unknown whether the increased neutrophil and interleukin (IL)-8 levels observed in induced sputum reflect systemic or local airway inflammation. We assessed the potential role of resident cells in intermittent hypoxia-induced airway inflammation. Methods Airway epithelial cells (AEC) and bronchial smooth muscle cells (BSMC) were exposed to intermittent hypoxia (IH) in vitro. Cell supernatants were assessed for matrix metalloproteinase, growth factor, and cytokine expression. The role of IH on neutrophil and BSMC migration capacities was evaluated, and the effect of supernatants from IH-exposed or control AEC was tested. Results Compared to normoxic conditions, 24 h of exposure to IH induced a significant increase of MMP-9 and MMP-2 expression and pro-MMP-9 activation ( p  
ISSN:1520-9512
1522-1709
DOI:10.1007/s11325-014-1019-4