DNA amplification polymorphisms of the cultivated mushroom Agaricus bisporus

• Published in: Applied and Environmental Microbiology 1992-09, Vol.58 (9), p.2971-2977
• Main Authors: Khush, R.S. (Monterey Laboratories, Watsonville, CA), Becker, E, Wach, M
• Format: Article
• Language: English
• Subjects: ADN; AGARICUS; Agaricus - classification; Agaricus - genetics; Agaricus - growth & development; Agaricus bisporus; Agronomy. Soil science and plant productions; Base Sequence; BIOCHIMIE; Biological and medical sciences; BIOQUIMICA; Biotechnology; Biotechnology & Applied Microbiology; Blotting, Southern; Crosses, Genetic; Deoxyribonucleic acid; DNA; DNA Fingerprinting; DNA, Fungal - isolation & purification; Fundamental and applied biological sciences. Psychology; Gene Amplification; Genetic engineering applications; Genetics; Genetics and breeding of economic plants; Life Sciences & Biomedicine; MARCADORES GENETICOS; MARQUEUR GENETIQUE; Microbiology; Molecular Sequence Data; Mushrooms; Plant breeding: fundamental aspects and methodology; POLIMORFISMO GENETICO; Polymorphism, Genetic; POLYMORPHISME GENETIQUE; Science & Technology; Species Specificity
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.58.9.2971-2977.1992

Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0 kbp. RAPD markers identified seven distinct genotypes among eight heterokaryotic strains; two of the commercial strains were shown to be related to each other through single-spore descent. Homokaryons recovered from protoplast regenerants of heterokaryotic strains carried a subset of the RAPD markers found in the heterokaryon, and both of the haploid nuclei from two heterokaryons were distinguishable. RAPD markers also served to verify the creation of a hybrid heterokaryon and to analyze meiotic progeny from this new strain: most of the basidiospores displayed RAPD fingerprints identical to that of the parental heterokaryon, although a few selected slow growers were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryotic basidiospores; crossover events between a RAPD marker locus and its respective centromere appeared to be infrequent. These results demonstrate that RAPD markers provide an efficient alternative for strain fingerprinting and a versatile toot for genetic studies and manipulations of A. bisporus