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MHC class II molecules, cathepsins, and La/SSB proteins in lacrimal acinar cell endomembranes
Departments of Physiology and Biophysics, Cell and Neurobiology, and Ophthalmology, School of Medicine, and Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, California 90033; and Institut für Physiologische Chemie, Fachbereich Medzin, Johanne...
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Published in: | American Journal of Physiology: Cell Physiology 1999-11, Vol.277 (5), p.C994-C1007 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Departments of Physiology and Biophysics, Cell and Neurobiology, and
Ophthalmology, School of Medicine, and Department of Pharmaceutical
Sciences, School of Pharmacy, University of Southern California, Los
Angeles, California 90033; and Institut für Physiologische
Chemie, Fachbereich Medzin, Johannes Gutenberg-Universität
Mainz, Mainz, Germany
Sjögren's
syndrome is a chronic autoimmune disease affecting the lacrimal glands
and other epithelia. It has been suggested that acinar cells of the
lacrimal glands provoke local autoimmune responses, leading to
Sjögren's syndrome when they begin expressing major
histocompatibility complex (MHC) class II molecules. We used isopycnic
centrifugation and phase partitioning to resolve compartments that
participate in traffic between the basolateral membranes
and the endomembrane system to test the hypothesis that MHC class II
molecules enter compartments that contain potential autoantigens, i.e.,
La/SSB, and enzymes capable of proteolytically processing autoantigen,
i.e., cathepsins B and D. A series of compartments identified as
secretory vesicle membranes, prelysosomes, and microdomains of the
trans-Golgi network involved in traffic to the basolateral membrane, to
the secretory vesicles, and to the prelysosomes were all prominent loci
of MHC class II molecules, La/SSB, and cathepsins B and D. These
observations support the thesis that lacrimal gland acinar cells that
have been induced to express MHC class II molecules function as
autoantigen processing and presenting cells.
Sjögren's syndrome; autoantigen processing and presentation; membrane traffic |
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ISSN: | 0363-6143 1522-1563 |
DOI: | 10.1152/ajpcell.1999.277.5.C994 |