Pinacidil suppresses contractility and preserves energy but glibenclamide has no effect during muscle fatigue

• Published in: American Journal of Physiology: Cell Physiology 2000-02, Vol.278 (2), p.C404-C416
• Main Authors: Matar, W, Nosek, T. M, Wong, D, Renaud, J.-M
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Adenosine Triphosphate - metabolism; Animals; Energy Metabolism - drug effects; Glyburide - pharmacology; Hypoglycemic Agents - pharmacology; Mice; Mice, Inbred Strains; Muscle Contraction - drug effects; Muscle Fatigue - drug effects; Muscle, Skeletal - chemistry; Muscle, Skeletal - drug effects; Muscle, Skeletal - physiology; Phosphocreatine - metabolism; Pinacidil - pharmacology; Potassium Channels - metabolism; Rubidium Radioisotopes; Space life sciences; Vasodilator Agents - pharmacology
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.2000.278.2.C404

1  Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5; and 2  Department of Physiology and Endocrinology, Medical College of Georgia, Augusta, Georgia 30912-3000 The effects of 10 µM glibenclamide, an ATP-sensitive K + (K ATP ) channel blocker, and 100 µM pinacidil, a channel opener, were studied to determine how the K ATP channel affects mouse extensor digitorum longus (EDL) and soleus muscle during fatigue. Fatigue was elicited with 200-ms-long tetanic contractions every second. Glibenclamide did not affect rate and extent of fatigue, force recovery, or 86 Rb + fractional loss. The only effects of glibenclamide during fatigue were: an increase in resting tension (EDL and soleus), a depolarization of the cell membrane, a prolongation of the repolarization phase of action potential, and a greater ATP depletion in soleus. Pinacidil, on the other hand, increased the rate but not the extent of fatigue, abolished the normal increase in resting tension during fatigue, enhanced force recovery, and increased 86 Rb + fractional loss in both the EDL and soleus. During fatigue, the decreases in ATP and phosphocreatine of soleus muscle were less in the presence of pinacidil. The glibenclamide effects suggest that fatigue, elicited with intermittent contractions, activates few K ATP channels that affect resting tension and membrane potentials but not tetanic force, whereas opening the channel with pinacidil causes a faster decrease in tetanic force, improves force recovery, and helps in preserving energy. skeletal muscle; tetanic force; resting potential; action potential; rubidium-86; phosphocreatine; extensor digitorum longus; adenosine 5'-triphosphate