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Effect of hepatic denervation on peripheral insulin sensitivity in conscious dogs

1  Department of Molecular Physiology and Biophysics and 2  Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 We tested the hypothesis that the loss of hepatic nerves decreases peripheral insulin sensitivity. Surgical hepatic denervation (DN)...

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Published in:American journal of physiology: endocrinology and metabolism 2002-02, Vol.282 (2), p.E286-E296
Main Authors: Moore, Mary Courtney, Satake, Shosuke, Baranowski, Bryan, Hsieh, Po-Shiuan, Neal, Doss W, Cherrington, Alan D
Format: Article
Language:English
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Summary:1  Department of Molecular Physiology and Biophysics and 2  Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 We tested the hypothesis that the loss of hepatic nerves decreases peripheral insulin sensitivity. Surgical hepatic denervation (DN) was performed in 22 dogs ~16 days before study; 7 dogs (Sham-Sal) had a sham procedure. A euglycemic hyperinsulinemic (1 mU · kg 1 · min 1 ; arterial insulin 35 ± 1 µU/ml in all dogs) clamp was performed in conscious dogs. From 0 to 90 min of the clamp, all dogs received the same treatment; then the DN dogs were divided into three groups. From 90 to 180 min, DN-PeA ( n  = 7) and DN-PoA ( n  = 7) groups received acetylcholine 2.5 µg · kg 1 · min 1 via peripheral or portal vein, respectively, and DN-Sal ( n  =   8) received no acetylcholine. During 150-180 min, the Sham-Sal, DN-Sal, DN-PeA, and DN-PoA groups exhibited glucose infusion rates of 12.4 ± 0.8, 9.3 ± 0.8 ( P
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.00201.2001