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Effect of hepatic denervation on peripheral insulin sensitivity in conscious dogs
1 Department of Molecular Physiology and Biophysics and 2 Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 We tested the hypothesis that the loss of hepatic nerves decreases peripheral insulin sensitivity. Surgical hepatic denervation (DN)...
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Published in: | American journal of physiology: endocrinology and metabolism 2002-02, Vol.282 (2), p.E286-E296 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 1 Department of Molecular Physiology and Biophysics and
2 Diabetes Research and Training Center, Vanderbilt
University School of Medicine, Nashville, Tennessee 37232
We tested the
hypothesis that the loss of hepatic nerves decreases peripheral insulin
sensitivity. Surgical hepatic denervation (DN) was performed in 22 dogs
~16 days before study; 7 dogs (Sham-Sal) had a sham procedure. A
euglycemic hyperinsulinemic (1 mU · kg 1 · min 1 ; arterial
insulin 35 ± 1 µU/ml in all dogs) clamp was performed in
conscious dogs. From 0 to 90 min of the clamp, all dogs received the
same treatment; then the DN dogs were divided into three groups. From
90 to 180 min, DN-PeA ( n = 7) and DN-PoA
( n = 7) groups received acetylcholine 2.5 µg · kg 1 · min 1 via
peripheral or portal vein, respectively, and DN-Sal ( n = 8) received no acetylcholine. During 150-180 min, the Sham-Sal, DN-Sal, DN-PeA, and DN-PoA groups exhibited glucose infusion rates of
12.4 ± 0.8, 9.3 ± 0.8 ( P |
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ISSN: | 0193-1849 1522-1555 |
DOI: | 10.1152/ajpendo.00201.2001 |