Optical mapping of activation patterns in an animal model of congenital heart block

1  Molecular and Cellular Cardiology Program, Veterans Affairs New York Harbor Health Care System, and 2  State University of New York Health Science Center, Brooklyn, New York 11209 Congenital heart block (CHB) is associated with high mortality and affects children of mothers with autoantibodies (I...

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Bibliographic Details
Published in:American journal of physiology. Heart and circulatory physiology 2001-04, Vol.280 (4), p.H1889-H1895
Main Authors: Restivo, Mark, Kozhevnikov, Dmitry O, Boutjdir, Mohamed
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Atrial Function, Right - physiology
Autoantibodies - blood
Bradycardia - immunology
Bradycardia - physiopathology
Disease Models, Animal
Electrocardiography - drug effects
Female
Heart - physiology
Heart - physiopathology
Heart Block - congenital
Heart Block - immunology
Heart Block - physiopathology
Humans
Immunoglobulin G - blood
Immunoglobulin G - pharmacology
In Vitro Techniques
Male
Perfusion
Pregnancy
Rabbits
Reference Values
Ribonucleoproteins - immunology
Sinoatrial Node - physiopathology
Ventricular Function, Right - physiology
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Summary:1  Molecular and Cellular Cardiology Program, Veterans Affairs New York Harbor Health Care System, and 2  State University of New York Health Science Center, Brooklyn, New York 11209 Congenital heart block (CHB) is associated with high mortality and affects children of mothers with autoantibodies (IgG) to ribonucleoproteins SSB/La and SSA/Ro. IgG from mothers of children with CHB (positive IgG) was used to assess activation patterns in both the right atrium (RA) and right ventricle (RV) of Langendorff-perfused young rabbit hearts. Optical action potentials (AP) were obtained by using a 124-site photodiode array with 4-[-[2-(di- n -butylamino)-6-naphthyl]vinyl]pyridinium. Optical APs were recorded to simultaneously image activation patterns from the RA and RV. Perfusion of positive IgG (800-1,200 µg/ml) resulted in sinus bradycardia and varying degrees of heart block. Activation maps revealed marked conduction delay at the sinoatrial junction but only minor changes in overall atrial and ventricular activation patterns. No conduction disturbances were seen in the presence of IgG from mothers with healthy children. In conclusion, besides atrioventricular (AV) block, positive IgG induces sinus bradycardia. These results establish that the sequelae of CHB are associated with impaired intrasinus and/or sinoatrial conduction. The findings raise the possibility that sinus bradycardia in the developing heart may indicate the potential for AV conduction disturbances. cardiac electrophysiology; autoantibodies; atrioventricular node
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.2001.280.4.h1889