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Modeling the interactions of particulates with epithelial lining fluid antioxidants
1 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn 10-747, Poland; 2 The Rayne Institute, St. Thomas' Hospital, London SE1 7EH; and 3 Cardiff School of Biosciences, Cardiff CF1 1ST, United Kingdom Oxidative stress may be a fundamental mode of injury ass...
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Published in: | American journal of physiology. Lung cellular and molecular physiology 1999-10, Vol.277 (4), p.719-L726 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 1 Institute of Animal
Reproduction and Food Research, Polish Academy of Sciences, Olsztyn
10-747, Poland; 2 The Rayne
Institute, St. Thomas' Hospital, London SE1 7EH; and
3 Cardiff School of Biosciences,
Cardiff CF1 1ST, United Kingdom
Oxidative stress may be a fundamental mode of
injury associated with inspired particles. To examine this, we
determined the ability of three carbon black particles (CBPs; M120,
M880, and R250) and two forms of silicon dioxide, amorphous (Cabosil)
and crystalline (DQ12) quartz, to deplete epithelium lining fluid antioxidant defenses. Single and composite antioxidant solutions of
uric acid, ascorbic acid (AA), and reduced glutathione (GSH) were
examined in the presence of particle concentrations of 150 µg/ml.
Uric acid was not depleted by any particle considered. AA was depleted
in a near-linear fashion with time by the three different CBPs;
however, AA depletion rates varied markedly with CBP type and decreased
in the presence of metal chelators. An initially high GSH depletion
rate was noted with all CBPs, and this was always accompanied by the
appearance of oxidized glutathione. Exposure to Cabosil or DQ12 did not
result in the loss of GSH. Together, these data demonstrate that
particle type, size, and surface area are all important factors when
considering particle-antioxidant interactions in the airways.
oxidative stress; particles; air pollution; ascorbic acid; reduced
glutathione |
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ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.1999.277.4.L719 |