Exogenous calmodulin potentiates vasodilation elicited by phospholipid-associated VIP in vivo

Departments of 1  Medicine, 3  Pharmaceutics and Pharmacodynamics, and 4  Bioengineering, University of Illinois at Chicago, and 2  West Side Department of Veterans Affairs Medical Center, Chicago, Illinois 60612 The purpose of this study was to determine whether exogenous calmodulin potentiates vas...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1999-05, Vol.276 (5), p.1359-R1365
Main Authors: Ikezaki, Hiroyuki, Patel, Manisha, Onyuksel, Hayat, Akhter, Syed R, Gao, Xiao-Pei, Rubinstein, Israel
Format: Article
Language:English
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Summary:Departments of 1  Medicine, 3  Pharmaceutics and Pharmacodynamics, and 4  Bioengineering, University of Illinois at Chicago, and 2  West Side Department of Veterans Affairs Medical Center, Chicago, Illinois 60612 The purpose of this study was to determine whether exogenous calmodulin potentiates vasoactive intestinal peptide (VIP)-induced vasodilation in vivo and, if so, whether this response is amplified by association of VIP with sterically stabilized liposomes. Using intravital microscopy, we found that calmodulin suffused together with aqueous and liposomal VIP did not potentiate vasodilation elicited by VIP in the in situ hamster cheek pouch. However, preincubation of calmodulin with liposomal, but not aqueous, VIP for 1 and 2   h and overnight at 4°C before suffusion significantly potentiated vasodilation ( P  
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.1999.276.5.r1359