Inhibition of Perforant Path Input to the CA1 Region by Serotonin and Noradrenaline

• Published in: Journal of neurophysiology 2005-08, Vol.94 (2), p.1413-1422
• Main Authors: Otmakhova, Nonna A, Lewey, Jennifer, Asrican, Brent, Lisman, John E
• Format: Article
• Language: English
• Subjects: Adrenergic alpha-Antagonists - pharmacology; Adrenergic beta-Antagonists - pharmacology; Animals; Animals, Newborn; Benzofurans - pharmacology; Dopamine - pharmacology; Electric Stimulation - methods; Excitatory Postsynaptic Potentials - drug effects; Excitatory Postsynaptic Potentials - physiology; Excitatory Postsynaptic Potentials - radiation effects; Hippocampus - cytology; Imidazoles - pharmacology; In Vitro Techniques; Neural Inhibition - drug effects; Neural Inhibition - physiology; Neural Inhibition - radiation effects; Neurons - drug effects; Neurons - physiology; Neurons - radiation effects; Norepinephrine - pharmacology; Patch-Clamp Techniques - methods; Perforant Pathway - drug effects; Perforant Pathway - physiology; Propranolol - pharmacology; Rats; Rats, Long-Evans; Serotonin - pharmacology; Serotonin Agents - pharmacology; Yohimbine - pharmacology
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00217.2005

Department of Biology and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts Submitted 1 March 2005; accepted in final form 8 May 2005 Bath-applied monoamines—dopamine (DA), serotonin (5-HT), and noradrenaline (NE)—strongly suppress the perforant path (PP) input to CA1 hippocampal region with very little effect on the Schaffer collaterals (SC) input. The effect of DA action on PP field excitatory postsynaptic potential (fEPSP) has been characterized in detail, but relatively little is known about the NE and 5-HT effects. Here we show that the maximal inhibition of the PP fEPSP by NE is 55%, whereas 5-HT inhibition is weaker ( 35%). The half-maximal inhibitory concentration of both 5-HT and NE is 1 µM. Neither NE nor 5-HT affected paired-pulse facilitation, suggesting that the effect is not presynaptic. This is in contrast to DA, which does have a presynaptic effect. The NE effect was blocked by 2 antagonists, whereas the 1 antagonist corynanthine and -antagonist propranolol were ineffective. The effect of 5-HT was mimicked by the agonist, 5-carboxamidotryptamine maleate (5-CT), and not affected by adrenergic and dopaminergic antagonists. To determine the 5-HT receptors involved, we tested a number of 5-HT antagonists, but none produced a complete suppression of the 5-HT effect. Of these, only the 5-HT 7 and 5-HT 2 antagonists produced weak but significant inhibition of 5-HT effect. We conclude that NE inhibits the PP fEPSP through postsynaptic action on 2 -adrenoceptors and that 5-HT 7 , 5-HT 2 , and some other receptor may be involved in 5-HT action in PP. Address for reprint requests and other correspondence: J. E. Lisman, Volen CCS, Brandeis Univ., 415 South St., Waltham, MA 02454 (E-mail: lisman{at}brandeis.edu )