Uteroplacental insufficiency induces site-specific changes in histone H3 covalent modifications and affects DNA-histone H3 positioning in day 0 IUGR rat liver

• Published in: Physiological genomics 2004-12, Vol.20 (1), p.108-116
• Main Authors: Fu, Qi, McKnight, Robert A, Yu, Xing, Wang, Laiyi, Callaway, Christopher W, Lane, Robert H
• Format: Article
• Language: English
• Subjects: Animals; Birth Weight; Blotting, Western; Carnitine O-Palmitoyltransferase - biosynthesis; Cell Nucleus - metabolism; Chromatin - chemistry; Chromatin - metabolism; Chromatin Immunoprecipitation; DNA - chemistry; Epigenesis, Genetic; Female; Fetal Growth Retardation - metabolism; Histones - chemistry; Histones - metabolism; Lysine - chemistry; Male; Phenotype; Placental Insufficiency - pathology; Polymerase Chain Reaction; Pregnancy; Pregnancy, Animal; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Transcription Factors - biosynthesis
• ISSN: 1094-8341; 1531-2267
• DOI: 10.1152/physiolgenomics.00175.2004

University of Utah School of Medicine, Department of Pediatrics, Division of Neonatology, Salt Lake City, Utah Uteroplacental insufficiency and subsequent intrauterine growth retardation (IUGR) increase the risk of adult onset insulin resistance and dyslipidemia in humans and rats. IUGR rats are further characterized by postnatal alterations in hepatic PPAR- coactivator (PGC-1) and carnitine-palmitoyl-transferase I (CPTI) expression, as well as overall hyperacetylation of histone H3. However, it is unknown whether the histone H3 hyperacetylation is site specific or relates to the changes in gene expression previously described in IUGR rats. We therefore hypothesized that uteroplacental insufficiency causes site-specific modifications in hepatic H3 acetylation and affects the association of acetylated histone H3 with PGC-1 and CPTI promoter sequences. Uteroplacental insufficiency was used to produce asymmetrical IUGR rats. IUGR significantly increased acetylation of H3 lysine-9 (H3/K9), lysine-14 (H3/K14), and lysine-18 (H3/K18) at day 0 of life, and these changes occurred in association with decreased nuclear protein levels of histone deacetylase 1 (HDAC1) and HDAC activity. Chromatin immunoprecipitation using acetyl-H3/K9 antibody and day 0 chromatin revealed that uteroplacental insufficiency affected the association between acetylated H3/K9 and the promoters of PGC-1 and CPTI, respectively, in IUGR liver. At day 21 of life, the neonatal pattern of H3 hyperacetylation persisted only in the IUGR males. We conclude that uteroplacental insufficiency increases H3 acetylation in a site-specific manner in IUGR liver and that these changes persist in male IUGR animals. The altered association of the PGC-1 and CPTI promoters with acetylated H3/K9 correlates with previous reports of IUGR altering the expression of these genes. We speculate that in utero alterations of chromatin structure contribute to fetal programming. epigenetics; Barker’s fetal origins of adult disease hypothesis; low birth weight