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Regulation of syntaxin1A–munc18 complex for SNARE pairing in HEK293 cells

The formation and dissolution of SNARE protein complexes is essential for Ca 2+ -triggered fusion of neurotransmitter-filled vesicles at the presynaptic membrane. Among the pre-synaptic SNARE proteins, the activation of the Q-SNARE syntaxin1A is a critical event for SNARE complex formation. Activati...

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Bibliographic Details
Published in:The Journal of physiology 2004-08, Vol.558 (3), p.857
Main Authors: Svetlana E. Gladycheva, Chi S. Ho, Yue Ying F. Lee, Edward L. Stuenkel
Format: Article
Language:English
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Summary:The formation and dissolution of SNARE protein complexes is essential for Ca 2+ -triggered fusion of neurotransmitter-filled vesicles at the presynaptic membrane. Among the pre-synaptic SNARE proteins, the activation of the Q-SNARE syntaxin1A is a critical event for SNARE complex formation. Activation requires syntaxin1A to transit from a munc18-bound non-interacting state to one competent for SNARE binding. The molecular mechanisms that regulate this transition remain unclear. The propensity of syntaxin1A to promote voltage-dependent steady-state inactivation of N-type Ca 2+ channels and accelerate their entry into inactivation was used in a heterologous cell expression system to elucidate regulation of syntaxin1A protein–protein interactions. We report that coexpression of munc18 eliminated the promoting effect of syntaxin1A on inactivation. This effect of munc18 was completely disrupted by coexpression of munc13-1, but not munc13-2 or munc13-3. Also, since expression of munc13-1 with syntaxin1A resulted in an inactivation phenotype identical to that of munc18 with syntaxin1A, the action of munc13-1 on the munc18–syntaxin1A complex was functionally unique and did not result from competitive binding interactions. Furthermore, munc13 expressed with syntaxin1A and munc18 promoted redistribution of a cytosolic SNAP25 mutant to the membrane, a result indicative of syntaxin1A–SNAP25 SNARE pairing. These data demonstrate an important role of munc13 to control the protein–protein interactions of syntaxin1A in vivo, and support munc13 as critical to dissociating syntaxin1A–munc18 complexes and making syntaxin1A available for SNARE interactions.
ISSN:0022-3751
1469-7793
DOI:10.1113/jphysiol.2004.067249